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          • Primary Antibodies ›
          • CD3e/CD3g Antibodies

          Invitrogen

          CD3e/CD3g Recombinant Rabbit Monoclonal Antibody (4F5G4)

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          Cite CD3e/CD3g Recombinant Rabbit Monoclonal Antibody (4F5G4)

          Additional Information:
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          • Antibody Testing Data (3)
          CD3e/CD3g Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          CD3e/CD3g Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          CD3e/CD3g Antibody (MA5-42744) in WB

          Western blot analysis of CD3e/CD3g in various lysates. Samples were incubated with CD3e/CD3g Monoclonal antibody (Product # MA5-42744) using a dilution of 1:2,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          CD3e/CD3g Antibody in Western Blot (WB)
          CD3e/CD3g Antibody in Flow Cytometry (Flow)
          CD3e/CD3g Antibody in Flow Cytometry (Flow)
          CD3e/CD3g Recombinant Rabbit Monoclonal Antibody (4F5G4)

          Product Details

          MA5-42744

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000-1:5,000
          -

          Flow Cytometry (Flow)

          1:500-1:1,000
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          4F5G4

          Immunogen

          Recombinant fusion protein containing a sequence corresponding to amino acids 1-207(CD3E)& 1-182(CD3G) of human CD3 epsilon&CD3 gamma Heterodimer
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.0 mg/mL

          Amount

          100 µg

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 0.05% BSA, 50% glycerol

          Contains

          0.09% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2911885

          Product Specific Information

          The target is usually found in the following locations: Cell membrane, Single-pass type I membrane protein, Membrane, Single-pass type I membrane protein.

          Immunogen sequence: MQSGTHWRVL GLCLLSVGVW GQDGNEEMGG ITQTPYKVSI SGTTVILTCP QYPGSEILWQ HNDKNIGGDE DDKNIGSDED HLSLKEFSEL EQSGYYVCYP RGSKPEDANF YLYLRARVCE NCMEMDVMSV ATIVIVDICI TGGLLLLVYY WSKNRKAKAK PVTRGAGAGG RQRGQNKERP PPVPNPDYEP IRKGQRDLYS GLNQRRI(CD 3E)&MEQGKG LAVLILAIIL LQGTLAQSIK GNHLVKVYDY QEDGSVLLTC DAEAKNITWF KDGKMIGFLT EDKKKWNLGS NAKDPRGMYQ CKGSQNKSKP LQVYYRMCQN CIELNAATIS GFLFAEIVSI FVLAVGVYFI AGQDGVRQSR ASDKQTLLPN DQLYQPLKDR EDDQYSHLQG NQLRRN(CD3 G)

          Target Information

          The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: CD3-epsilon; CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell receptor T3 gamma chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; unnamed protein product

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          Gene Aliases: CD3-GAMMA; CD3E; CD3epsilon; CD3G; CD3GAMMA; IMD17; IMD18; T3E; T3G; TCRE

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          UniProt ID: (Human) P07766, (Human) P09693

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          Entrez Gene ID: (Human) 916, (Human) 917

          View more View less

          Function(s)
          transmembrane signaling receptor activity protein binding SH3 domain binding protein kinase binding receptor signaling complex scaffold activity T cell receptor binding identical protein binding
          Process(es)
          adaptive immune response immune system process establishment or maintenance of cell polarity cell surface receptor signaling pathway protein transport T cell activation positive thymic T cell selection gamma-delta T cell activation alpha-beta T cell activation T cell receptor signaling pathway protein-containing complex assembly regulation of lymphocyte apoptotic process positive regulation of cell-matrix adhesion positive regulation of T cell anergy T cell anergy transmembrane receptor protein tyrosine kinase signaling pathway signal complex assembly G-protein coupled receptor signaling pathway smoothened signaling pathway regulation of signal transduction positive regulation of gene expression negative regulation of gene expression dendrite development calcium-mediated signaling cerebellum development T cell costimulation positive regulation of interferon-gamma production positive regulation of interleukin-2 production positive regulation of interleukin-4 production positive regulation of cell-cell adhesion mediated by integrin CD4-positive, alpha-beta T cell proliferation T cell proliferation positive regulation of T cell proliferation regulation of apoptotic process positive regulation of MAPK cascade negative thymic T cell selection negative regulation of smoothened signaling pathway lymphocyte activation positive regulation of calcium-mediated signaling positive regulation of T cell activation apoptotic signaling pathway positive regulation of CD4-positive, alpha-beta T cell proliferation
          It has to be done as per old AB suggested Products section.

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