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Invitrogen

CD4/CD8 Antibody Cocktail, FITC, PE

View all (3) CD4/CD8 antibodies

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Product Details

MA5-16802

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

Neat
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Cocktail

Type

Antibody

Clone

LT8/RPA-T4

Immunogen

Normal human blood lymphocytes, Human PHA blasts

Conjugate

FITC , PE FITC , PE FITC , PE

Form

Lyophilized

Concentration

Conc. Not Determined

Purification

Ion-exchange chromatography, Protein G

Storage buffer

PBS with 1% BSA, 5% sucrose

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538285

Product Specific Information

MA5-16802 is suitable for use a negative control for measurement of non-specific binding of mouse monoclonals of isotype IgG1 in rat tissue in a dual labelling technique using FITC and R-Phycoerythrin fluorochromes. This antibody contains a monoclonal mixture of FITC-conjugated Mouse IgG1 RPE-conjugated Mouse IgG1.

Reconstitute with 0.5 mL of distilled water. After reconstitution, store product undiluted at 4°C in the dark.

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti Human CD8:FITC/CD4:RPE Dual Color reeagent may be used to simultaneously to identify CD4 and CD8 positive cells.

CD4, clone LT8 conjugated to FITC, is purified by ion exchange chromatography, and CD8, clone RPA-T4 conjugated to RPE, is prepared by affinity chromatography on Protein G and then purified by gel-filtration to separate the unconjugated material.

Target Information

CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lymphocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV. The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a corepressor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains, or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8a molecule; CD8alpha; CD8b; CD8b molecule; CD8beta; cell surface glycoprotein T8 precursor; Leu-2; Leu2 T-lymphocyte antigen; Ly-3 homolog; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein (CD8-beta) precursor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell differentiation antigen Leu-2/T8; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T4 surface glycoprotein precursor; T8 T-cell antigen; unnamed protein product

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Gene Aliases: CD4; CD4mut; CD8; CD8A; CD8alpha; CD8B; CD8B1; CD8beta; IMD116; IMD79; Leu-3; LEU2; Ly-3; LY3; LYT3; MAL; OKT4D; p32; P37; T4

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UniProt ID: (Human) P01730, (Human) P01732, (Human) P10966

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Entrez Gene ID: (Human) 920, (Human) 925, (Human) 926

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Function(s)
virus receptor activity transmembrane signaling receptor activity receptor binding extracellular matrix structural constituent protein binding zinc ion binding lipid binding coreceptor activity immunoglobulin binding enzyme binding protein kinase binding MHC class II protein complex binding signaling receptor activity interleukin-16 binding interleukin-16 receptor activity MHC class II protein binding identical protein binding protein homodimerization activity protein tyrosine kinase binding MHC class I protein complex binding MHC class I protein binding
Process(es)
adaptive immune response immune system process immune response transmembrane receptor protein tyrosine kinase signaling pathway T cell activation regulation of immune response T cell receptor signaling pathway positive regulation of protein phosphorylation positive regulation of immune system process cell adhesion signal transduction cell surface receptor signaling pathway enzyme linked receptor protein signaling pathway positive regulation of calcium ion transport into cytosol calcium-mediated signaling T cell differentiation macrophage differentiation response to estradiol maintenance of protein location in cell positive regulation of interleukin-2 production response to vitamin D positive regulation of kinase activity helper T cell enhancement of adaptive immune response interleukin-15-mediated signaling pathway positive regulation of T cell proliferation positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAPK cascade T cell selection response to ethanol positive regulation of monocyte differentiation positive regulation of protein kinase activity positive regulation of transcription, DNA-templated positive regulation of viral entry into host cell viral entry into host cell defense response to Gram-negative bacterium positive regulation of calcium-mediated signaling regulation of T cell activation positive regulation of T cell activation positive regulation of multicellular organismal process regulation of calcium ion transport positive regulation of ERK1 and ERK2 cascade cellular response to granulocyte macrophage colony-stimulating factor stimulus response to methamphetamine hydrochloride cellular response to ionomycin T cell mediated immunity antigen processing and presentation cytotoxic T cell differentiation
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