|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||PBS with 4mg/ml BSA, sucrose|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Allophycocyanin (APC) is a stable and highly soluble phycobiliprotein that provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing exceptional quantum yields and molar extinction coefficients.
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.
Analyte Specific Reagent
Dynamic change in natural killer cell type in the human ocular mucosa in situ as means of immune evasion by adenovirus infection.
MHCD4505-4 was used in flow cytometry to investigate the dynamics and characteristics of natural killer cell types in the human ocular mucosal surface in situ during infection with group D human adenoviruses.
|Yawata N,Selva KJ,Liu YC,Tan KP,Lee AW,Siak J,Lan W,Vania M,Arundhati A,Tong L,Li J,Mehta JS,Yawata M||Mucosal immunology (9:159)||2016|
Dielectrophoretic capture and genetic analysis of single neuroblastoma tumor cells.
MHCD4505-4 was used in flow cytometry to describe a novel, non-invasive, and adaptable method for the capture and genetic analysis of single tumor cells from cancer patients.
|Carpenter EL,Rader J,Ruden J,Rappaport EF,Hunter KN,Hallberg PL,Krytska K,O'Dwyer PJ,Mosse YP||Frontiers in oncology (4:null)||2014|
Blood cell-derived induced pluripotent stem cells free of reprogramming factors generated by Sendai viral vectors.
MHCD4505-4 was used in flow cytometry to describe a protocol to generated iPSCs from adult mobilized CD34+ and peripheral blood mononuclear cells.
|Ye L,Muench MO,Fusaki N,Beyer AI,Wang J,Qi Z,Yu J,Kan YW||Stem cells translational medicine (2:558)||2013|
Tyrosine kinase inhibitor insensitivity of non-cycling CD34+ human acute myeloid leukaemia cells with FMS-like tyrosine kinase 3 mutations.
MHCD4505-4 was used in flow cytometry to study non-cycling acute myeloid leukemia cells.
|Alvares CL,Schenk T,Hulkki S,Min T,Vijayaraghavan G,Yeung J,Gonzalez D,So CW,Greaves M,Titley I,Bartolovic K,Morgan G||British journal of haematology (154:457)||2011|
Phenotypic and molecular characterization of CD103+ CD4+ T cells in bronchoalveolar lavage from patients with interstitial lung diseases.
MHCD4505-4 was used in flow cytometry to test if CD103 has a regulatory role in the microenvironment of the epithelial cell layer.
|Braun RK,Foerster M,Grahmann PR,Haefner D,Workalemahu G,Kroegel C||Cytometry. Part B, Clinical cytometry (54:19)||2003|