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|Tested species reactivity||Human|
|Published species reactivity||Human , Mouse|
|Host / Isotype||Mouse / IgG1|
|Storage buffer||PBS with 4mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 5 publications below|
Our Pacific Orange™ dye is optimally excited by the violet laser, and it is recommended that a 575, 585 or 600 nm band pass filter be used for optimal detection. Pacific Blue™ and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm and 551 nm, respectively, facilitating two-color analysis.
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.
Analyte Specific Reagent
Dynamic change in natural killer cell type in the human ocular mucosa in situ as means of immune evasion by adenovirus infection.
MHCD4530 was used in flow cytometry to investigate the dynamics and characteristics of natural killer cell types in the human ocular mucosal surface in situ during infection with group D human adenoviruses.
|Yawata N,Selva KJ,Liu YC,Tan KP,Lee AW,Siak J,Lan W,Vania M,Arundhati A,Tong L,Li J,Mehta JS,Yawata M||Mucosal immunology (9:159)||2016|
Plasmacytoid dendritic cells suppress HIV-1 replication but contribute to HIV-1 induced immunopathogenesis in humanized mice.
MHCD4530 was used in flow cytometry to study the role of plasmacytoid dendritic cells in human immunodeficiency virus type 1 infection and pathogenesis
|Li G,Cheng M,Nunoya J,Cheng L,Guo H,Yu H,Liu YJ,Su L,Zhang L||PLoS pathogens (10:null)||2014|
Immune response to JC virus T antigen in patients with and without colorectal neoplasia.
MHCD4530 was used in flow cytometry to study the cell mediated immune response to JCV T-Ag in patients with colorectal adenomatous polyps or cancers
|Butcher LD,Garcia M,Arnold M,Ueno H,Goel A,Boland CR||Gut microbes (5:468)||2014|
Characterization of human antiviral adaptive immune responses during hepatotropic virus infection in HLA-transgenic human immune system mice.
MHCD4530 was used in flow cytometry to investigate the role of HLA molecules during infection using humanized mice
|Billerbeck E,Horwitz JA,Labitt RN,Donovan BM,Vega K,Budell WC,Koo GC,Rice CM,Ploss A||Journal of immunology (Baltimore, Md. : 1950) (191:1753)||2013|
In vitro immunomodulation of a whole blood IFN-γ release assay enhances T cell responses in subjects with latent tuberculosis infection.
MHCD4530 was used in flow cytometry to discuss the use of PPR ligands as adjuvants and the use of interferon-gamma release assays as a diagnostic tool
|Gaur RL,Suhosk MM,Banaei N||PloS one (7:null)||2012|
CD45 antigen, T200 glycoprotein, T200 leukocyte common antigen, protein tyrosine phosphatase, receptor type, c polypeptide, receptor-type tyrosine-protein phosphatase C, B220, CD45, CD45R, GP180, L-CA, LCA, LY5, protein tyrosine phosphatase, receptor type, c polypeptide
B220, T200, LY5, LCA, L-CA, CD45, GP180, CD45R, RP11-553K8.4