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CD49e (Integrin alpha 5) Monoclonal Antibody (eBioSAM-1 (SAM-1, SAM1)), Alexa Fluor™ 488, eBioscience™
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-Flow-20170216130641.jpg?time=20220826 "CD49e (Integrin alpha 5) Antibody in Flow Cytometry (Flow)")
FIGURE: 1 / 2
CD49e (Integrin alpha 5) Antibody (53-0496-42) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD3 APC (Product # 17-0038-42) and Mouse IgG1 kappa Isotype Control Alexa Fluor® 488 (Product # 53-4714-42) (left) or Anti-Human CD49e (Integrin alpha 5) Alexa Fluor® 488 (right). Cells in the lymphocyte gate were used for analysis.
-Flow-20170216130641.jpg?time=20220826)
-Flow-20170216130641.jpg?time=20220826?time=20220826 "CD49e (Integrin alpha 5) Antibody (53-0496-42) in Flow")
Product Details
53-0496-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG2b
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBioSAM-1 (SAM-1, SAM1)
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The eBioSAM-1 monoclonal antibody reacts with human integrin alpha 5, also known as fibronectin receptor alpha chain, very late activation antigen 5 alpha, and CD49e. Integrins are composed of an alpha chain and a beta chain, which non-covalently associate to form the functional integrin. Integrin heterodimers participate in cell surface-mediated signaling and adhesion functions. Integrin alpha 5 undergoes post-translational cleavage in its extracellular domain to yield disulfide linked light and heavy chains that join with Integrin beta 1 (CD29) to form the fibronectin receptor, also known as the very late activation antigen-5 (VLA-5) complex. Integrin alpha 5 is expressed on thymocytes, T cells, monocytes, platelets, early B cells, and activated B cells.
Applications Reported: This eBioSAM-1 (SAM-1, SAM1) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBioSAM-1 (SAM-1, SAM1) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: CD49 antigen-like family member E; CD49e; Fibronectin receptor subunit alpha; fibronectin receptor, alpha polypeptide; fibronectin receptor, alpha subunit; Integrin alpha-5; Integrin alpha-F; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); ITGA5/SLC9B1 fusion; surface glycoprotein receptor; unnamed protein product; very late activation protein 5, alpha subunit; VLA-5
Gene Aliases: CD49e; FNRA; ITGA5; VLA-5; VLA5A
UniProt ID: (Human) P08648
Entrez Gene ID: (Human) 3678
virus receptor activity
receptor binding
epidermal growth factor receptor binding
platelet-derived growth factor receptor binding
integrin binding
calcium ion binding
protein binding
signaling receptor activity
vascular endothelial growth factor receptor 2 binding
metal ion binding
cell adhesion molecule binding
angiogenesis
formation of primary germ layer
cell-substrate junction assembly
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
cell-matrix adhesion
integrin-mediated signaling pathway
female pregnancy
memory
tissue development
positive regulation of cell-substrate adhesion
response to muscle activity
CD40 signaling pathway
positive regulation of cell migration
positive regulation of vascular endothelial growth factor receptor signaling pathway
cell-substrate adhesion
cell adhesion mediated by integrin
cell-cell adhesion mediated by integrin
heterotypic cell-cell adhesion
wound healing, spreading of epidermal cells
endodermal cell differentiation
regulation of angiogenesis
viral entry into host cell
cell-cell adhesion
positive regulation of vascular endothelial growth factor signaling pathway
positive regulation of sprouting angiogenesis
negative regulation of anoikis
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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