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Invitrogen
CD49f Polyclonal Antibody
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FIGURE: 1 / 4
CD49f Antibody (PA5-12334) in ICC/IF
Immunocytochemistry analysis of CD49f in HeLa cells. Samples were incubated with CD49f polyclonal antibody (Product # PA5-12334) using a dilution of 1:200 for 2 h at room temperature followed by Alexa Fluor® 488 conjugated donkey anti-rabbit at a dilution of 1:1,000 for 1hr. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.2%, 30 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/mL, 5 min). Note the highly specific localization of the ITGA6 mainly to the nucleus.
Product Details
PA5-12334
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 1043-1072 amino acids from isoform 2 of human ITGA6
Conjugate
Form
Liquid
Concentration
0.48 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with chicken based on sequence homology.
Target Information
CDf (Integrin alpha-6, ITGA6) is the integrin alpha chain alpha 6 protein. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signaling. Two transcript variants encoding different isoforms have been found for CDf. Functionally, integrins are cell surface receptors that interact with the extracellular matrix and mediate intracellular signals that affect cellular shape, mobility, and progression through the cell cycle in response to the extracellular matrix. Integrin receptors are composed of alpha and beta subunits, and form structural and functional linkages between the ECM (extra-cellular matrix) and intracellular cytoskeletal linker proteins. Signaling mediated by Intergrin/ECM interactions are also integrated with cellular responses to growth factor signaling to regulate cellular proliferation, cytoskeletal reorganization and other responses necessary for cellular survival. CDf is predominantly expressed by epithelia, and plays a critical structural role in the hemidesmosome. More than 18 alpha and 8 beta subunits with numerous splice variant isoforms have been identified in mammals. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD49 antigen-like family member F; CD49f; DKFZp686J01244; FLJ18737; integrin alpha 6; Integrin alpha-6; integrin alpha6B; integrin, alpha 6; VLA-6
Gene Aliases: CD49f; ITGA6; ITGA6B; VLA-6
UniProt ID: (Human) P23229
Entrez Gene ID: (Human) 3655
cell-substrate junction assembly
cell-matrix adhesion
integrin-mediated signaling pathway
ectodermal cell differentiation
positive regulation of cell-cell adhesion
extracellular matrix organization
hemidesmosome assembly
cell-substrate adhesion
cellular response to extracellular stimulus
cell adhesion mediated by integrin
nail development
positive regulation of phosphorylation
positive regulation of apoptotic process
positive regulation of GTPase activity
skin development
positive regulation of transcription from RNA polymerase II promoter
filopodium assembly
digestive tract development
brown fat cell differentiation
leukocyte migration
renal system development
amelogenesis
cell-cell adhesion
negative regulation of extrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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