|Tested species reactivity||Human|
|Published species reactivity||Human, Rhesus monkey|
|Host / Isotype||Mouse / IgG1|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The CD5, a transmembrane protein, is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. CD5 is expressed by many T cell leukemia, lymphomas, and activated T cells. Occasionally, CD5 antigen is also expressed on a subset of B cells. Mantle cell lymphomas (same as diffuse centrocytic lymphomas) are CD5+ while the follicle center cell lymphoma are CD5-.
Analyte Specific Reagent
|Rhesus monkey||Not Cited||
Double-positive CD21+CD27+ B cells are highly proliferating memory cells and their distribution differs in mucosal and peripheral tissues.
MHCD0500 was used in flow cytometry to identify and characterize memory B cells in normal rhesus macaques.
|Das A,Xu H,Wang X,Yau CL,Veazey RS,Pahar B||PloS one (6:null)||2011|
Comparison of methods for determining zeta-chain associated protein - 70 (ZAP-70) expression in patients with B-cell chronic lymphocytic leukemia (B-CLL).
MHCD0500 was used in flow cytometry to investigate novel monoclonal antibodies to ZAP-70 to detect B-cell chronic lymphocytic leukemia
|Bojarska-Junak A,Giannopoulos K,Kowal M,Dmoszy¿ska A,Roli¿ski J||Cytometry. Part B, Clinical cytometry (70:293)||2006|
Separation of functional subsets of human T cells by a monoclonal antibody.
MHCD0500 was used in flow cytometry to characterize OKT4 positive T cells.
|Reinherz EL,Kung PC,Goldstein G,Schlossman SF||Proceedings of the National Academy of Sciences of the United States of America (76:4061)||1979|
Flow cytometry in the bone marrow staging of mature B-cell neoplasms.
MHCD0500 was used in flow cytometry to ask if flow cytometry can be used for the detection of bone marrow involvement in the different types of B-cell malignant neoplasms.
|Stacchini A,Demurtas A,Godio L,Martini G,Antinoro V,Palestro G||Cytometry. Part B, Clinical cytometry (54:10)||2003|