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Invitrogen
CD55 Polyclonal Antibody
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FIGURE: 1 / 1
CD55 Antibody (PA5-89530) in WB
Western blot analysis of CD55 in extracts of A-549 cells. Samples were incubated with CD55 Polyclonal antibody (Product # PA5-89530) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Product Details
PA5-89530
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein (or fragment)
Conjugate
Form
Liquid
Concentration
1.86 mg/mL
Amount
186 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: PDVPNAQPAL EGRTSFPEDT VITYKCEESF VKIPGEKDSV ICLKGSQWSD IEEFCNRSCE VPTRLNSASL KQPYITQNYF PVGTVVEYEC RPGYRREPSL SPKLTCLQNL KWSTAVEFCK KKSCPNPGEI RNGQIDVPGG ILFGATISFS CNTGYKLFGS TSSFCLISGS SVQWSDPLPE CREIYCPAPP QIDNGIIQGE RDHYGYRQSV TYACNKGFTM IGEHSIYCTV NNDEGEWSGP PPECRGKSLT SKVPPTVQKP T; Positive Samples: A-549; Cellular Location: Cell membrane, GPI-anchor, Lipid-anchor, Single-pass type I membrane protein
Target Information
CD55 (decay-accelerating factor, DAF) is a GPI-anchored membrane glycoprotein that protects autologous cells from classical and alternative pathway of complement cascade. Bidirectional interactions between CD55 and CD97 are involved in T cell regulation and CD55 can regulate complement when bound to CD97. In tumors, CD55 promotes neoangiogenesis, tumorigenesis, invasiveness and evasion of apoptosis. CD55 is a 70 kDa glycoprotein (in erythrocytes) anchored in the membrane by glycosylphosphatidylinositol tail. In other cells, the apparent molecular weight is somewhat larger for CD55 and has a substantial content of O-glycans. CD55 binds to activated C4b or C3b complement fragments on the cell surface, preventing the assembly and accelerating the decay of both classical and alternative pathways. CD55 carries the Cromer related blood group antigens. Two alternatively spliced transcripts encoding CD55 have been identified, and the predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. CD55 has been studied to have a role in prostate cancer growth and survival.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD55; CD55 antigen; CD55 molecule, decay accelerating factor for complement (Cromer blood group); Complement decay-accelerating factor; Complement decay-accelerating factor, GPI-anchored; complement-glycosylphosphatidylinositol; Cromer blood group; Cromer blood group antigen; DAF-GPI; decay accelerating factor 1; decay-accelerating factor precursor; GPI anchor addition signal; Rh blood group D antigen; RP11-357P18.1; unnamed protein product
Gene Aliases: CD55; Cd55a; CHAPLE; CR; CROM; DAF; Daf-GPI; Daf1; GPI-DAF; TC
UniProt ID: (Human) P08174, (Mouse) Q61475
Entrez Gene ID: (Human) 1604, (Mouse) 13136
immune system process
complement activation, classical pathway
regulation of complement activation, classical pathway
negative regulation of catalytic activity
innate immune response
viral entry into host cell
regulation of lymphocyte mediated immunity
positive regulation of T cell cytokine production
regulation of adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains
positive regulation of cytosolic calcium ion concentration
regulation of complement activation
regulation of lipopolysaccharide-mediated signaling pathway
respiratory burst
negative regulation of complement activation
regulation of complement-dependent cytotoxicity
positive regulation of CD4-positive, alpha-beta T cell activation
positive regulation of CD4-positive, alpha-beta T cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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