|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a, kappa|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 5 publications below|
NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig)-like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins.
Analyte Specific Reagent
Variation in Gamma-Globin Expression before and after Induction with Hydroxyurea Associated with BCL11A, KLF1 and TAL1.
MHCD5600 was used in flow cytometry to elucidate regulation of γ-globin expression in F-cells.
|Grieco AJ,Billett HH,Green NS,Driscoll MC,Bouhassira EE||PloS one (10:null)||2015|
Molecular characterization of human 4Ig-B7-H3, a member of the B7 family with four Ig-like domains.
MHCD5600 was used in flow cytometry to characterize B7-H3.
|Steinberger P,Majdic O,Derdak SV,Pfistershammer K,Kirchberger S,Klauser C,Zlabinger G,Pickl WF,Stöckl J,Knapp W||Journal of immunology (Baltimore, Md. : 1950) (172:2352)||2004|
LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling.
MHCD5600 was used in flow cytometry to describe a new raft-associated adaptor protein, Lck-interacting molecule, expressed predominantly in T lymphocytes.
|Brdicková N,Brdicka T,Angelisová P,Horváth O,Spicka J,Hilgert I,Paces J,Simeoni L,Kliche S,Merten C,Schraven B,Horejsí V||The Journal of experimental medicine (198:1453)||2003|
Trafficking machinery of NKT cells: shared and differential chemokine receptor expression among V alpha 24(+)V beta 11(+) NKT cell subsets with distinct cytokine-producing capacity.
MHCD5600 was used in flow cytometry to report differential expression of chemokine receptors among NKT cell subsets.
|Kim CH,Johnston B,Butcher EC||Blood (100:11)||2002|
Identity of Leu-19 (CD56) leukocyte differentiation antigen and neural cell adhesion molecule.
MHCD5600 was used in immunoprecipitation to propose that the Leu-19 antigen on leukocytes contributes to cell adhesion and is analogous to N-CAM on neural cells.
|Lanier LL,Testi R,Bindl J,Phillips JH||The Journal of experimental medicine (169:2233)||1989|