|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Storage buffer||PBS with BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.
CD69 is a 55-70 kD glycoprotein that under reducing conditions dissociates in 3 components (gp 26,28,34), differentially glycosylated forms of a 21kD protein. CD69 is an early activation antigen also called Activation Inducer Molecule (AIM) that is very early, before antigens such as CD25 and CD71 are expressed strongly on T-cells after activation. CD69 is also expressed by activated B-lymphocytes.
Analyte Specific Reagent
Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.
MHCD6901 was used in flow cytometry develop an HLA-A2-specific chimeric antigen receptor and use it to generate alloantigen-specific human T regulatory cells
|MacDonald KG,Hoeppli RE,Huang Q,Gillies J,Luciani DS,Orban PC,Broady R,Levings MK||The Journal of clinical investigation (126:1413)||2016|
Immunological/virological peripheral blood biomarkers and distinct patterns of sleeping quality in chronic hepatitis C patients.
MHCD6901 was used in flow cytometry to identify immunological/virological biomarkers associated with sleep quality in patients with chronic hepatitis C.
|de Almeida CM,de Lima TA,Castro DB,Torres KL,da Silva Braga W,Peruhype-Magalhães V,Teixeira-Carvalho A,Martins-Filho OA,Malheiro A||Scandinavian journal of immunology (73:486)||2011|
Human CD4+CD25+ regulatory cells have marked and sustained effects on CD8+ T cell activation.
MHCD6901 was used in flow cytometry to study the interactions between CD4(+)CD25(+) T cells and human CD8(+) T cells.
|Câmara NO,Sebille F,Lechler RI||European journal of immunology (33:3473)||2003|