|Tested species reactivity||Human|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Mouse / IgG2a|
|Storage buffer||PBS with 4mg/ml BSA, sucrose|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 2 publications below|
R-phycoerythrin (PE) is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing an exceptional quantum yields and molar extinction coefficients.
CD7 (gp40, Leu9) is a member of the immunoglobulin gene superfamily. Its N-terminal amino acids 1-107 are highly homologous to Ig kappa-L chains whereas the carboxy-terminal region of the extracellular domain is proline-rich and has been postulated to form a stalk from which the Ig domain projects. CD7 is expressed on the majority of immature and mature T-lymphocytes, and T cell leukemia. It is also found on natural killer cells, a small subpopulation of normal B cells and on malignant B cells. Cross-linking surface CD7 positively modulates T cell and NK cell activity as measured by calcium fluxes, expression of adhesion molecules, cytokine secretion and proliferation. CD7 associates directly with phosphoinositol 3'-kinase. CD7 ligation induces production of D-3 phosphoinositides and tyrosine phosphorylation.
Analyte Specific Reagent
New IDH1 mutant inhibitors for treatment of acute myeloid leukemia.
MHCD0704 was used in flow cytometry to assess the effects of allosteric inhibitors on different mutant forms of isocitrate dehydrogenase 1 in leukemia
|Okoye-Okafor UC,Bartholdy B,Cartier J,Gao EN,Pietrak B,Rendina AR,Rominger C,Quinn C,Smallwood A,Wiggall KJ,Reif AJ,Schmidt SJ,Qi H,Zhao H,Joberty G,Faelth-Savitski M,Bantscheff M,Drewes G,Duraiswami C,Brady P,Groy A,Narayanagari SR,Antony-Debre I,Mitchell K,Wang HR,Kao YR,Christopeit M,Carvajal L,Barreyro L,Paietta E,Makishima H,Will B,Concha N,Adams ND,Schwartz B,McCabe MT,Maciejewski J,Verma A,Steidl U||Nature chemical biology (11:878)||2015|
Flow cytometry significantly improves the diagnostic value of fine needle aspiration cytology of lymphoproliferative lesions of salivary glands.
MHCD0704 was used in flow cytometry to compare fine needle aspiration cytology with flow cytometry immunophenotyping for the diagnosis of lymphoproliferative processes in the salivary glands.
|Stacchini A,Aliberti S,Pacchioni D,Demurtas A,Isolato G,Gazzera C,Veltri A,Maletta F,Molinaro L,Novero D||Cytopathology : official journal of the British Society for Clinical Cytology (25:231)||2014|