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Invitrogen
CD73 Polyclonal Antibody
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Product Details
PA5-82585
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human NT5E. Recombinant protein control fragment (Product #RP-90963).
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Amount
20 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: EFDNGVEGLI EPLLKEAKFP ILSANIKAKG PLASQISGLY LPYKVLPVGD EVVGIVGYTS KETPFLSNPG TNLVFEDEIT ALQPEVDKLK TLNVNKIIAL GHSGFEMDKL IAQK
Highest antigen sequence indentity to the following orthologs: Rat - 92%, Mouse - 90%.
Target Information
CD73, also known as Ecto-5-prime-nucleotidase or 5-prime-ribonucleotide phosphohydrolase, is an enzyme that catalyzes the conversion of purine 5-prime mononucleotides to nucleosides at neutral pH, with AMP as its preferred substrate. It is composed of a dimer of two identical 70 kDa subunits, externally bound to the plasma membrane via a glycosyl phosphatidyl inositol linkage. CD73 serves as a marker of lymphocyte differentiation and is expressed on a subset of lymphocytes, increasing during lymphocyte maturation. It is found on memory CD4 T cells, which resemble uncommitted primed precursor helper cells (Thpp) capable of differentiating into Th1 or Th2 cells, and is also present on regulatory T cells. CD73 deficiency is associated with various immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes, distinguishable from CD73 by their substrate affinities, requirement for divalent magnesium ions, activation by ATP, and inhibition by inorganic phosphate. The CD73 gene is localized to chromosome 6q14-q21, and defects in this gene can lead to conditions such as calcification of joints and arteries, and intestinal tuberculosis. Additionally, two transcript variants encoding different isoforms of CD73 have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5'-deoxynucleotidase; 5'-NT; 5'-nucleotidase; CD73; Ecto-5'-nucleotidase; IMP-specific 5'-nucleotidase; Purine 5-Prime-Nucleotidase; Thymidylate 5'-phosphatase; unnamed protein product
Gene Aliases: CALJA; CD73; E5NT; eN; eNT; NT; NT5; NT5E; NTE
UniProt ID: (Human) P21589
Entrez Gene ID: (Human) 4907
AMP catabolic process
DNA metabolic process
leukocyte cell-cell adhesion
nucleotide catabolic process
response to ATP
ADP catabolic process
ATP metabolic process
adenosine biosynthetic process
negative regulation of inflammatory response
calcium ion homeostasis
inhibition of non-skeletal tissue mineralization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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