Invitrogen

CD73 Monoclonal Antibody (7G2)

CD73 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

CD73 Antibody (41-0200) in ICC/IF

Immunofluorescence analysis of CD73 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD73 Monoclonal Antibody (7G2) (Product # 41-0200) at a concentration of 1 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 co... View More

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CD73 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across the cell lines owing to their inherent genetic constitution. Relative expression of CD73 was observed in U-87 MG, Jurkat, MDA-MB-231 and SK-MEL-31 cell lines in comparison to THP-1 and HeLa using Anti-CD73 Monoclonal Antibody (7G2) (Product # 41-0200) in Western Blot. {RE}

Product Details

41-0200

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:100

View 4 publications 4 publications

Flow Cytometry (Flow)

Assay-dependent

View 7 publications 7 publications

ELISA (ELISA)

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Functional Assay (Functional)

Assay-dependent

Inhibition Assays (IA)

Assay-dependent

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

7G2

Immunogen

Highly purified human placental CD73 protein

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533492

Target Information

CD73, also known as Ecto-5-prime-nucleotidase or 5-prime-ribonucleotide phosphohydrolase, is an enzyme that catalyzes the conversion of purine 5-prime mononucleotides to nucleosides at neutral pH, with AMP as its preferred substrate. It is composed of a dimer of two identical 70 kDa subunits, externally bound to the plasma membrane via a glycosyl phosphatidyl inositol linkage. CD73 serves as a marker of lymphocyte differentiation and is expressed on a subset of lymphocytes, increasing during lymphocyte maturation. It is found on memory CD4 T cells, which resemble uncommitted primed precursor helper cells (Thpp) capable of differentiating into Th1 or Th2 cells, and is also present on regulatory T cells. CD73 deficiency is associated with various immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes, distinguishable from CD73 by their substrate affinities, requirement for divalent magnesium ions, activation by ATP, and inhibition by inorganic phosphate. The CD73 gene is localized to chromosome 6q14-q21, and defects in this gene can lead to conditions such as calcification of joints and arteries, and intestinal tuberculosis. Additionally, two transcript variants encoding different isoforms of CD73 have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Thermostable Basic Fibroblast Growth Factor Enhances the Production and Activity of Human Wharton's Jelly Mesenchymal Stem Cell-Derived Extracellular Vesicles.

International journal of molecular sciences

Park S,Kim S,Lim K,Shin Y,Song K,Kang GH,Kim DY,Shin HC,Cho SG

Published figure using CD73 monoclonal antibody (Product # 41-0200) in Flow Cytometry

Fri Nov 17 00:00:00 UTC 2023

Sarcococca saligna fabricated gold nanoparticles alleviated in vitro oxidative stress and inflammation in human adipose-derived stem cells.

Journal of biomedical materials research. Part B, Applied biomaterials

Naseer N,Mustafa MM,Latief N,Fazal N,Tariq M,Afreen A,Yaqub F,Riazuddin S

41-0200 was used in Immunocytochemistry to conclude that S. saligna bio fabricated GNPs priming may improve the therapeutic potential of hADMSCs against chronic inflammatory problems by regulating NF-κB pathway.

Fri Dec 01 00:00:00 UTC 2023

A Protocol for the Isolation, Culture, and Cryopreservation of Umbilical Cord-Derived Canine Mesenchymal Stromal Cells: Role of Cell Attachment in Long-Term Maintenance.

Stem cells and development

Wright A,Snyder L,Knights K,He H,Springer NL,Lillich J,Weiss ML

Published figure using CD73 monoclonal antibody (Product # 41-0200) in Flow Cytometry

Mon Jun 01 00:00:00 UTC 2020

Mitochondrial Transfer of Wharton's Jelly Mesenchymal Stem Cells Eliminates Mutation Burden and Rescues Mitochondrial Bioenergetics in Rotenone-Stressed MELAS Fibroblasts.

Oxidative medicine and cellular longevity

Lin TK,Chen SD,Chuang YC,Lan MY,Chuang JH,Wang PW,Hsu TY,Wang FS,Tsai MH,Huang ST,Wang XW,Tsai PC,Lin HY,Liou CW

41-0200 was used in Flow cytometry/Cell sorting to determine the therapeutic effect of Wharton's jelly mesenchymal stem cells on mitochondiral dysfunction in MELAS patients.

Fri Dec 13 00:00:00 UTC 2019

Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells.

Biological research

Madrigal A,Tan L,Zhao Y

41-0200 was used in Immunocytochemistry to show that regulators of G protein signalling proteins may play multifaceted supportive or conflicting roles during human adipogenesis and osteogenesis.

Tue Dec 26 00:00:00 UTC 2017

Extracellular Vesicles from Adipose-Derived Mesenchymal Stem/Stromal Cells Accelerate Migration and Activate AKT Pathway in Human Keratinocytes and Fibroblasts Independently of miR-205 Activity.

Stem cells international

Ferreira ADF,Cunha PDS,Carregal VM,da Silva PC,de Miranda MC,Kunrath-Lima M,de Melo MIA,Faraco CCF,Barbosa JL,Frezard F,Resende V,Rodrigues MA,de Goes AM,Gomes DA

41-0200 was used in Flow cytometry/Cell sorting to explore the role of extracellular vesicles from adipose tissue mesenchymal stem/stromal cells in promoting wound healing, and the proliferation and migration of keratinocytes and fibroblasts.

Wed Sep 30 00:00:00 UTC 2020

Characterization of Tunneling Nanotubes in Wharton's jelly Mesenchymal Stem Cells. An Intercellular Exchange of Components between Neighboring Cells.

Stem cell reviews and reports

Sanchez V,Villalba N,Fiore L,Luzzani C,Miriuka S,Boveris A,Gelpi RJ,Brusco A,Poderoso JJ

410200 was used in immunocytochemistry to describe the types, structure, components, dynamics and functionality of the tunneling nanotubes bridging neighboring human umbilical cord mesenchymal stem cells obtained from Wharton's jelly

Tue Aug 01 00:00:00 UTC 2017

Blood-based markers of efficacy and resistance to cetuximab treatment in metastatic colorectal cancer: results from CALGB 80203 (Alliance).

Cancer medicine

Hatch AJ,Sibley AB,Starr MD,Brady JC,Jiang C,Jia J,Bowers DL,Pang H,Owzar K,Niedzwiecki D,Innocenti F,Venook AP,Hurwitz HI,Nixon AB

410200 was used in ELISA to find prognostic and predictive biomarkers for patients with colorectal cancer treated with cetuximab

Thu Sep 01 00:00:00 UTC 2016

Mesenchymal markers on human adipose stem/progenitor cells.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Zimmerlin L,Donnenberg VS,Rubin JP,Donnenberg AD

Published figure using CD73 monoclonal antibody (Product # 41-0200) in Flow Cytometry

Tue Jan 01 00:00:00 UTC 2013

Mesenchymal markers on human adipose stem/progenitor cells.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Zimmerlin L,Donnenberg VS,Rubin JP,Donnenberg AD

Published figure using CD73 monoclonal antibody (Product # 41-0200) in Flow Cytometry

Tue Jan 01 00:00:00 UTC 2013

Dengue virus type 2 modulates endothelial barrier function through CD73.

The American journal of tropical medicine and hygiene

Patkar C,Giaya K,Libraty DH

41-0200 was used in Flow Cytometry to examine the modulation of endothelial barrier function in dengue virus type 2 infections using primary human umbilical vein endothelial cells.

Tue Jan 01 00:00:00 UTC 2013

Morphological and immunocytochemical characteristics indicate the yield of early progenitors and represent a quality control for human mesenchymal stem cell culturing.

Journal of anatomy

Haasters F,Prall WC,Anz D,Bourquin C,Pautke C,Endres S,Mutschler W,Docheva D,Schieker M

Published figure using CD73 monoclonal antibody (Product # 41-0200) in Immunofluorescence

Fri May 01 00:00:00 UTC 2009

Production and characterization of monoclonal antibodies to the glycosyl phosphatidylinositol-anchored lymphocyte differentiation antigen ecto-5'-nucleotidase (CD73).

Tissue antigens

Thomson LF,Ruedi JM,Glass A,Moldenhauer G,Moller P,Low MG,Klemens MR,Massaia M,Lucas AH

Mon Jan 01 00:00:00 UTC 1990

Production and characterization of monoclonal antibodies to the glycosyl phosphatidylinositol-anchored lymphocyte differentiation antigen ecto-5'-nucleotidase (CD73).

Tissue antigens

Thomson LF,Ruedi JM,Glass A,Moldenhauer G,Moller P,Low MG,Klemens MR,Massaia M,Lucas AH

Mon Jan 01 00:00:00 UTC 1990

Production and characterization of monoclonal antibodies to the glycosyl phosphatidylinositol-anchored lymphocyte differentiation antigen ecto-5'-nucleotidase (CD73).

Tissue antigens

Thomson LF,Ruedi JM,Glass A,Moldenhauer G,Moller P,Low MG,Klemens MR,Massaia M,Lucas AH

Mon Jan 01 00:00:00 UTC 1990

Bioinformatics

Protein Aliases: 5'-deoxynucleotidase; 5'-NT; 5'-nucleotidase; 5'-nucleotidase, ecto (CD73); CD73; Ecto-5'-nucleotidase; IMP-specific 5'-nucleotidase; Purine 5-Prime-Nucleotidase; Thymidylate 5'-phosphatase

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Gene Aliases: CALJA; CD73; E5NT; eN; eNT; NT; NT5; NT5E; NTE

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UniProt ID: (Human) P21589

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Entrez Gene ID: (Human) 4907

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Function(s)

nucleotide binding ferrous iron binding 5'-nucleotidase activity phosphodiesterase

Process(es)

purine nucleotide biosynthetic process purine nucleotide catabolic process AMP catabolic process DNA metabolic process leukocyte cell-cell adhesion brain development response to aluminum ion dephosphorylation adenosine biosynthetic process pyrimidine nucleoside catabolic process positive regulation of lipid biosynthetic process negative regulation of inflammatory response

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD73 Monoclonal Antibody (7G2)

CD73 Antibody (41-0200) in ICC/IF

Product Details

41-0200

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Functional Assay (Functional)

Inhibition Assays (IA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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