Invitrogen

CD73 Monoclonal Antibody (7G2)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

3 Published Figures

12 References

View all (65) CD73 antibodies

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Protocols

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CD73 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of CD73 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD73 Monoclonal Antibody (7G2) (Product # 41-0200) at a concentration of 1 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing membrane localization. Panel e represents HeLa cells showing no expression of CD73. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

CD73 Antibody in Western Blot (WB) — Western blot was performed using Anti-CD73 Monoclonal Antibody (7G2) (Product # 41-0200) and a 63 kDa band corresponding to CD73 was observed across the cell lines tested except THP-1 and HeLa. Whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), THP-1 (Lane 2), Jurkat (Lane 3), MDA-MB-231 (Lane 4), SK-MEL-31 (Lane 5) and HeLa (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody at a concentration of 1 µg/mL and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

CD73 Antibody in Flow Cytometry (Flow) — Human peripheral blood lymphocytes stained with Mouse anti-CD73 monoclonal antibody (Product # 41-0200).

CD73 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across the cell lines owing to their inherent genetic constitution. Relative expression of CD73 was observed in U-87 MG, Jurkat, MDA-MB-231 and SK-MEL-31 cell lines in comparison to THP-1 and HeLa using Anti-CD73 Monoclonal Antibody (7G2) (Product # 41-0200) in Western Blot. {RE}

Product Details

41-0200

Applications	Tested Dilution	Publications
Western Blot (WB)	1 µg/mL	-
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Frozen) (IHC (F))	Assay-dependent	-
Immunocytochemistry (ICC/IF)	1:100	View 3 publications 3 publications
Flow Cytometry (Flow)	Assay-dependent	View 5 publications 5 publications
ELISA (ELISA)	-	View 1 publication 1 publication
Immunoprecipitation (IP)	Assay-dependent	View 1 publication 1 publication
Functional Assay (FN)	Assay-dependent	-
Inhibition Assays (IA)	Assay-dependent	-

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Mouse / IgG2a, kappa
Class	Monoclonal
Type	Antibody
Clone	7G2
Immunogen	Highly purified human placental CD73 protein
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4
Contains	0.1% sodium azide
Storage conditions	-20°C
RRID	AB_2533492

Target Information

CD73 (Ecto-5-prime-nucleotidase, 5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. CD73 consists of a dimer of 2 identical 70 kDa subunits bound externally to the plasma membrane by a glycosyl phosphatidyl inositol linkage. CD73 is used as a marker of lymphocyte differentiation. Consequently, a deficiency of CD73 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from CD73 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. The CD73 gene has been localized to 6q14-q21 by immunofluorescence and a study of a panel of human x mouse hybrids that contained fragments of chromosome 6 as translocations. Defects in the CD73 gene can lead to the calcification of joints and arteries, and intestinal tuberculosis. Two transcript variants encoding different isoforms of CD73 have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Extracellular Vesicles from Adipose-Derived Mesenchymal Stem/Stromal Cells Accelerate Migration and Activate AKT Pathway in Human Keratinocytes and Fibroblasts Independently of miR-205 Activity.

Stem cells international

Ferreira ADF,Cunha PDS,Carregal VM,da Silva PC,de Miranda MC,Kunrath-Lima M,de Melo MIA,Faraco CCF,Barbosa JL,Frezard F,Resende V,Rodrigues MA,de Goes AM,Gomes DA

41-0200 was used in Flow cytometry/Cell sorting to explore the role of extracellular vesicles from adipose tissue mesenchymal stem/stromal cells in promoting wound healing, and the proliferation and migration of keratinocytes and fibroblasts.

Wed Sep 30 00:00:00 EDT 2020

Mitochondrial Transfer of Wharton's Jelly Mesenchymal Stem Cells Eliminates Mutation Burden and Rescues Mitochondrial Bioenergetics in Rotenone-Stressed MELAS Fibroblasts.

Oxidative medicine and cellular longevity

Lin TK,Chen SD,Chuang YC,Lan MY,Chuang JH,Wang PW,Hsu TY,Wang FS,Tsai MH,Huang ST,Wang XW,Tsai PC,Lin HY,Liou CW

41-0200 was used in Flow cytometry/Cell sorting to determine the therapeutic effect of Wharton's jelly mesenchymal stem cells on mitochondiral dysfunction in MELAS patients.

Fri Dec 13 00:00:00 EST 2019

Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells.

Biological research

Madrigal A,Tan L,Zhao Y

41-0200 was used in Immunocytochemistry to show that regulators of G protein signalling proteins may play multifaceted supportive or conflicting roles during human adipogenesis and osteogenesis.

Tue Dec 26 00:00:00 EST 2017

Characterization of Tunneling Nanotubes in Wharton's jelly Mesenchymal Stem Cells. An Intercellular Exchange of Components between Neighboring Cells.

Stem cell reviews and reports

Sanchez V,Villalba N,Fiore L,Luzzani C,Miriuka S,Boveris A,Gelpi RJ,Brusco A,Poderoso JJ

410200 was used in immunocytochemistry to describe the types, structure, components, dynamics and functionality of the tunneling nanotubes bridging neighboring human umbilical cord mesenchymal stem cells obtained from Wharton's jelly

Tue Aug 01 00:00:00 EDT 2017

Blood-based markers of efficacy and resistance to cetuximab treatment in metastatic colorectal cancer: results from CALGB 80203 (Alliance).

Cancer medicine

Hatch AJ,Sibley AB,Starr MD,Brady JC,Jiang C,Jia J,Bowers DL,Pang H,Owzar K,Niedzwiecki D,Innocenti F,Venook AP,Hurwitz HI,Nixon AB

410200 was used in ELISA to find prognostic and predictive biomarkers for patients with colorectal cancer treated with cetuximab

Thu Sep 01 00:00:00 EDT 2016

Mesenchymal markers on human adipose stem/progenitor cells.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Zimmerlin L,Donnenberg VS,Rubin JP,Donnenberg AD

41-0200 was used in immunohistochemistry to identify the mesenchymal markers on human adipose stem/progenitor cells

Tue Jan 01 00:00:00 EST 2013

Mesenchymal markers on human adipose stem/progenitor cells.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Zimmerlin L,Donnenberg VS,Rubin JP,Donnenberg AD

41-0200 was used in immunohistochemistry to identify the mesenchymal markers on human adipose stem/progenitor cells

Tue Jan 01 00:00:00 EST 2013

Dengue virus type 2 modulates endothelial barrier function through CD73.

The American journal of tropical medicine and hygiene

Patkar C,Giaya K,Libraty DH

41-0200 was used in Flow Cytometry to examine the modulation of endothelial barrier function in dengue virus type 2 infections using primary human umbilical vein endothelial cells.