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Invitrogen
CD81 Polyclonal Antibody
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FIGURE: 1 / 7
CD81 Antibody (PA5-79004) in ICC/IF
Immunofluorescence analysis of CD81 antigen was performed using 70% confluent log phase MCF7 cells (a), MCF7 treated with Protein transport inhibitor (PTI) (1X for 4hrs) (b), and Hep G2 treated with Protein transport inhibitor (PTI) (1X for 4hrs) (c). The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for overnight at room temperature. The cells were labeled with CD81 Polyclonal Antibody (Product # PA5-79004) at 5 µg/mL concentration in 0.1% BSA, incuba... View More
Product Details
PA5-79004
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived mouse TAPA1 recombinant protein (Position: K116-K201).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
0.9mg NaCl, 0.2mg Na2PO4, 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat brain tissue, mouse ovary tissue, mouse brain tissue, mouse NIH/3T3 whole cell. IHC: rat liver tissue.
Target Information
CD81 (TAPA-1, target of anti-proliferative antibody-1) is a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T cells. In muscles, CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus. Like members of the tetraspanin family that include CD9, CD37, CD53, CD63, and CD82, CD81 is a cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD81 is a cell surface glycoprotein that is known to complex with integrins. CD81 appears to promote muscle cell fusion and support myotube maintenance. CD81 associates with CD19, CD21, Leu 13, and integrins on cell membrane and acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas. CD81 is also localized in the tumor-suppressor gene region and is a candidate gene for malignancies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 26 kDa cell surface protein TAPA-1; CD 81 antigen; CD81; CD81 antigen; CD81 antigen (target of antiproliferative antibody 1); Target of the antiproliferative antibody 1; Tetraspanin28
Gene Aliases: Cd81; Tapa-1; Tapa1; Tspan28
UniProt ID: (Mouse) P35762
Entrez Gene ID: (Rat) 25621, (Mouse) 12520
activation of MAPK activity
phosphatidylinositol biosynthetic process
protein localization
positive regulation of cell proliferation
response to wounding
positive regulation of B cell proliferation
regulation of growth
regulation of cell proliferation
positive regulation of 1-phosphatidylinositol 4-kinase activity
phosphatidylinositol metabolic process
positive regulation of peptidyl-tyrosine phosphorylation
regulation of cell motility
positive regulation of cell growth
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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