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Invitrogen
CD86 Polyclonal Antibody
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FIGURE: 1 / 3
CD86 Antibody (PA5-114995) in ICC/IF
Immunocytochemistry analysis of CD86 in A549 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114995). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
Product Details
PA5-114995
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
C. elegans,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CD86(Accession P42081), corresponding to amino acid residues E52-I102.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total CD86.
Target Information
CD86, along with CD80, is a member of the B7 family of costimulatory molecules and plays a crucial role in T cell activation and immune response regulation. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells, and its expression is upregulated on B cells through various stimuli, including the BCR complex, CD40, and certain cytokine receptors. As a type I membrane protein and member of the immunoglobulin superfamily, CD86 serves as a ligand for the T cell surface proteins CD28 and CTLA-4 (CD152). The interaction between CD86 and CD28 provides a costimulatory signal essential for T cell activation during antigen presentation, while binding with CTLA-4 negatively regulates T cell activation, diminishing the immune response. This interaction is critical for T-B cell crosstalk, T cell costimulation, autoantibody production, and Th2-mediated Ig production. The kinetics of CD86 upregulation upon stimulation suggest its significant contribution during the primary phase of an immune response. CD86 and CD80 have distinct roles in T helper cell differentiation, and insufficient co-stimulation involving these molecules can induce tolerance. Alternative splicing of CD86 results in two transcript variants encoding different isoforms, with additional variants described but not fully sequenced. Dysfunction in CD86 is associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Activation B7-2 antigen; B lymphocyte activation antigen B72; B-lymphocyte activation antigen B7-2; B70; BU63; CD28 antigen ligand 2; CD86; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CTLA-4 counter-receptor B7.2; early T cell costimulatory molecule-1; Early T-cell co-stimulatory molecule 1; Early T-cell costimulatory molecule 1; ETC-1; FUN-1; MGC34413; T-lymphocyte activation antigen CD86
Gene Aliases: B7; B7-2; B7.2; B70; Cd28l2; CD28LG2; CD86; CLS1; ETC-1; LAB72; Ly-58; Ly58; MB7; MB7-2; TS/A-2
UniProt ID: (Human) P42081, (Mouse) P42082
Entrez Gene ID: (Human) 942, (Mouse) 12524
response to yeast
adaptive immune response
T cell proliferation involved in immune response
negative regulation of T cell anergy
immune response
cell-cell signaling
aging
positive regulation of cell proliferation
regulation of phosphatidylinositol 3-kinase signaling
T cell costimulation
toll-like receptor 3 signaling pathway
response to interferon-gamma
positive regulation of activated T cell proliferation
T cell activation
B cell activation
response to drug
myeloid dendritic cell differentiation
positive regulation of lymphotoxin A biosynthetic process
positive regulation of interleukin-2 biosynthetic process
positive regulation of interleukin-4 biosynthetic process
positive regulation of T-helper 2 cell differentiation
positive regulation of transcription, DNA-templated
viral entry into host cell
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
defense response to virus
cellular response to lipopolysaccharide
cellular response to metal ion
cellular response to cytokine stimulus
toll-like receptor signaling pathway
immune system process
positive regulation of T cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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