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Invitrogen
CD86 Polyclonal Antibody
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FIGURE: 1 / 3
CD86 Antibody (PA5-79007) in IHC (P)
Immunohistochemistry (Paraffin) analysis of CD86 in paraffin-embedded section of human tonsil tissue using CD86 Polyclonal Antibody (Product # PA5-79007). Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with the primary antibody at a 2 µg/mL dilution overnight at 4°C. ... View More
Product Details
PA5-79007
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human CD86 recombinant protein (Position: A24-F329).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Raji whole cell, human Daudi whole cell, mouse spleen tissue, mouse thymus tissue, mouse J774A1 whole cell, mouse Ana-1 whole cell. IHC: human tonsil tissue.
Target Information
CD86, along with CD80, is a member of the B7 family of costimulatory molecules and plays a crucial role in T cell activation and immune response regulation. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells, and its expression is upregulated on B cells through various stimuli, including the BCR complex, CD40, and certain cytokine receptors. As a type I membrane protein and member of the immunoglobulin superfamily, CD86 serves as a ligand for the T cell surface proteins CD28 and CTLA-4 (CD152). The interaction between CD86 and CD28 provides a costimulatory signal essential for T cell activation during antigen presentation, while binding with CTLA-4 negatively regulates T cell activation, diminishing the immune response. This interaction is critical for T-B cell crosstalk, T cell costimulation, autoantibody production, and Th2-mediated Ig production. The kinetics of CD86 upregulation upon stimulation suggest its significant contribution during the primary phase of an immune response. CD86 and CD80 have distinct roles in T helper cell differentiation, and insufficient co-stimulation involving these molecules can induce tolerance. Alternative splicing of CD86 results in two transcript variants encoding different isoforms, with additional variants described but not fully sequenced. Dysfunction in CD86 is associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activation B7-2 antigen; B lymphocyte activation antigen B72; B-lymphocyte activation antigen B7-2; B70; BU63; CD28 antigen ligand 2; CD86; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CD86 v6; CTLA-4 counter-receptor B7.2; early T cell costimulatory molecule-1; Early T-cell co-stimulatory molecule 1; Early T-cell costimulatory molecule 1; ETC-1; FUN-1; MGC34413; T-lymphocyte activation antigen CD86; unnamed protein product
Gene Aliases: B7; B7-2; B7.2; B70; Cd28l2; CD28LG2; CD86; CLS1; ETC-1; LAB72; Ly-58; Ly58; MB7; MB7-2; TS/A-2
UniProt ID: (Human) P42081, (Mouse) P42082
Entrez Gene ID: (Human) 942, (Mouse) 12524
adaptive immune response
immune system process
positive regulation of immunoglobulin production
immune response
signal transduction
cell surface receptor signaling pathway
positive regulation of cell proliferation
T cell costimulation
positive regulation of interleukin-2 production
positive regulation of interleukin-4 production
positive regulation of lymphotoxin A production
positive regulation of T cell proliferation
T cell activation
B cell activation
negative regulation of T cell proliferation
positive regulation of T-helper 2 cell differentiation
positive regulation of transcription, DNA-templated
lymphocyte activation
viral entry into host cell
cellular response to lipopolysaccharide
positive regulation of NIK/NF-kappaB signaling
toll-like receptor signaling pathway
negative regulation of T cell anergy
positive regulation of activated T cell proliferation
defense response to virus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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