|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||B-lymphoblastoid cell line ARH 77|
|Storage buffer||PBS with 4mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 4 publications below|
Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined.
Analyte Specific Reagent
Susceptibility of immature and mature Langerhans cell-type dendritic cells to infection and immunomodulation by human cytomegalovirus.
MHCD8601 was used in flow cytometry to report that CD34(+) progenitor cell-derived Langerhans cells-type DCs exhibit a differentiation state-dependent susceptibility to CMV infection.
|Hertel L,Lacaille VG,Strobl H,Mellins ED,Mocarski ES||Journal of virology (77:7563)||2003|
CD80 and CD86 C domains play an important role in receptor binding and co-stimulatory properties.
MHCD8601 was used in flow cytometry to elucidate the importance of C domains in the functioning of CD80 and CD86.
|Vasu C,Wang A,Gorla SR,Kaithamana S,Prabhakar BS,Holterman MJ||International immunology (15:167)||2003|
CD40 ligand-stimulated B cell precursor leukemic cells elicit interferon-gamma production by autologous bone marrow T cells in childhood acute lymphoblastic leukemia.
MHCD8601 was used in flow cytometry to test if CD40L activates the antigen-presenting cell capacity of B cell precursor acute lymphoblastic leukemia cells.
|Todisco E,Gaipa G,Biagi E,Bonamino M,Gramigna R,Introna M,Biondi A||Leukemia (16:2046)||2002|
Human CD4(+)CD25(+) cells: a naturally occurring population of regulatory T cells.
MHCD8601 was used in flow cytometry to characterize CD4(+)CD25(+) human T cells.
|Ng WF,Duggan PJ,Ponchel F,Matarese G,Lombardi G,Edwards AD,Isaacs JD,Lechler RI||Blood (98:2736)||2001|