|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||B-lymphoblastoid cell line ARH 77|
|Storage buffer||PBS with sucrose, 4mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 2 publications below|
Allophycocyanin (APC) is a stable and highly soluble phycobiliprotein that provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing exceptional quantum yields and molar extinction coefficients.
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined.
Analyte Specific Reagent
Antitumor immunity triggered by melphalan is potentiated by melanoma cell surface-associated calreticulin.
MHCD8605 was used in flow cytometry to study the role of melanoma cell surface-associated calreticulin in melphalan-induced antitumor immunity.
|Dudek-Peri¿ AM,Ferreira GB,Muchowicz A,Wouters J,Prada N,Martin S,Kiviluoto S,Winiarska M,Boon L,Mathieu C,van den Oord J,Stas M,Gougeon ML,Golab J,Garg AD,Agostinis P||Cancer research (75:1603)||2015|
Concurrent MEK and autophagy inhibition is required to restore cell death associated danger-signalling in Vemurafenib-resistant melanoma cells.
MHCD8605 was used in flow cytometry to study the role of autophagy in melanoma
|Martin S,Dudek-Peri¿ AM,Maes H,Garg AD,Gabrysiak M,Demirsoy S,Swinnen JV,Agostinis P||Biochemical pharmacology (93:290)||2015|