Invitrogen
Applications | Tested Dilution | Publications |
---|---|---|
Immunohistochemistry (IHC) |
- | View 3 publications 3 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
Assay-Dependent | - |
Immunocytochemistry (ICC/IF) |
Assay-Dependent | - |
Product Specifications | |
---|---|
Species Reactivity |
Human |
Published species |
Human |
Host/Isotype |
Mouse / IgG2a, kappa |
Recommended Isotype Control |
Mouse IgG2a kappa Isotype Control (eBM2a), eFluor™ 660, eBioscience™ |
Class |
Monoclonal |
Type |
Antibody |
Clone |
AMC908 |
Conjugate |
eFluor™ 660
|
Excitation/Emission Profile |
View spectra |
Form |
Liquid |
Concentration |
0.2 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.2 |
Contains |
0.09% sodium azide |
Storage conditions |
4° C, store in dark, DO NOT FREEZE! |
RRID |
AB_2574148 |
Description: This AMC908 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alphaalpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells.
Applications Reported: This AMC908 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy.
Applications Tested: This AMC908 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen
Gene Aliases: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37
UniProt ID: (Human) P01732, (Human) P10966
Entrez Gene ID: (Human) 925, (Human) 926
Molecular Function:
defense/immunity protein
immunoglobulin receptor superfamily
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