Invitrogen

CD8a Monoclonal Antibody (AMC908), eFluor™ 660, eBioscience™

Product Image Gallery

CD8a Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of formalin-fixed paraffin embedded human tonsil using 5 µg/mL of Mouse IgG2a, K Isotype Control eFluor® 660 (left) or 5 µg/mL of Anti-Human CD8a eFluor® 660 (right). Nuclei are stained with DAPI.

CD8a Antibody in Immunohistochemistry (IHC) — Fig. 5 Heatmaps of cell populations from lung cancer and tonsil tissues using k-means clustering demonstrates distinct cell immune populations with expected patterns of biomarker expression. ( a ) Heatmap of the expression of Keratin, alphaSMA, and CD45 in all cells that were collected from LUNG-1-LN, LUNG-2-BR, LUNG-3-PR, and TONSIL-1 using k-means clustering. Each row is a cluster. The last column in each heat map shows the probability density function (pdf) plot showing the fit of each cell within the cluster, with the x-axis denoting the frequency of cells and y-axis denoting the Euclidean distance of the cell from the centroid of the cluster. The black vertical bars mark the immune cluster with high CD45 expression. ( b , c ) Heatmaps showing the expression of seven lymphocyte markers (CD45, CD3D, CD8A, CD4, CD20, PD1, FOXP3) from the cells within the CD45 high cluster from panel (a). ( b ) Each row represents protein marker expression data from a single cell or ( c ) each row represents a cluster. Note that fluorescence intensity values were log transformed and normalized between -1 to 1 as indicated by the color bar. ( d ) Galleries of immunofluorescence images of representative cells from each cluster in ( c ). (Scale bar: 5 um).

Product Details

50-0008-80

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	Assay-Dependent	-
Immunocytochemistry (ICC/IF)	Assay-Dependent	-

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Mouse / IgG2a, kappa
Recommended Isotype Control	Mouse IgG2a kappa Isotype Control (eBM2a), eFluor™ 660, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	AMC908
Conjugate	eFluor™ 660 Alexa Fluor 488 Biotin Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2574148

Product Specific Information

Description: This AMC908 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alphaalpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells.

Applications Reported: This AMC908 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy.

Applications Tested: This AMC908 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Vascular Disease and Thrombosis in SARS-CoV-2-Infected Rhesus Macaques.

Cell

Aid M,Busman-Sahay K,Vidal SJ,Maliga Z,Bondoc S,Starke C,Terry M,Jacobson CA,Wrijil L,Ducat S,Brook OR,Miller AD,Porto M,Pellegrini KL,Pino M,Hoang TN,Chandrashekar A,Patel S,Stephenson K,Bosinger SE,Andersen H,Lewis MG,Hecht JL,Sorger PK,Martinot AJ,Estes JD,Barouch DH

50-0008 was used in Immunohistochemistry to suggest a model in which critical interactions between inflammatory and thrombosis pathways lead to SARS-CoV-2-induced vascular disease.

Wed Nov 25 00:00:00 EST 2020

Highly multiplexed immunofluorescence images and single-cell data of immune markers in tonsil and lung cancer.

Scientific data

Rashid R,Gaglia G,Chen YA,Lin JR,Du Z,Maliga Z,Schapiro D,Yapp C,Muhlich J,Sokolov A,Sorger P,Santagata S

Published figure using CD8a monoclonal antibody (Product # 50-0008-82) in Immunohistochemistry

Tue Dec 17 00:00:00 EST 2019

Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes.

eLife

Lin JR,Izar B,Wang S,Yapp C,Mei S,Shah PM,Santagata S,Sorger PK

Published figure using CD8a monoclonal antibody (Product # 50-0008-82) in Immunofluorescence

Wed Jul 11 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

Gene Aliases: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

UniProt ID: (Human) P01732, (Human) P10966

Entrez Gene ID: (Human) 925, (Human) 926

Molecular Function: defense/immunity protein immunoglobulin receptor superfamily