Invitrogen

CD8a Monoclonal Antibody (RPA-T8), eFluor 450, eBioscience™

34 Published Figures

21 References

View all (275) CD8 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

CD8a Antibody in Flow Cytometry (Flow) — Staining of normal human peripheral blood cells with Anti-Human CD3 APC (Product # 17-0038-42) and 0.25 µg of Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (left) or Anti-Human CD8a eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis.

CD8a Antibody — FIG. 4. Capacity of iPSC-NCCs to suppress activation of T cells. (A) PBMCs (a healthy donor) in the presence of anti-CD3 and anti-CD28 antibodies were cocultured with iPSC-NCCs for 3 days. After 3 days, the PBMCs exposed to iPSC-NCCs were harvested for Ki-67 FACS analysis. Numbers in the FACS dot plots indicate double-positive cells (eg, CD3-Ki-67). These data are representative of three experiments. (B) Percentages of the proliferating T cells ( double-positive cells in A) were also examined. Data are the mean +- SD of three experiments. * P < 0.05, compared to the two groups. NS, not significant. (C) FACS density plots represent the expression of IFN-gamma on CD4 + T cells in the presence of iPSC-NCCs or control iPSCs. PBMCs were stained with anti-CD4 and anti-IFN-gamma. Numbers in the density plots indicate the percentage of cells double positive for CD4/IFN-gamma. These data are representative of three experiments. (D) Percentages of the IFN-gamma secreting CD4 + T cells ( double-positive cells in C) were also examined. Data are the mean +- SD of 3 experiments. * P < 0.05, compared to the two groups. NS, not significant. (E) Concentrations of IFN-gamma in supernatant (MLR plus iPSC-NCCs) were examined by human IFN-gamma ELISA. We also collected control samples: MLR without iPSC-NCCs and MLR plus iPSCs. Data are the mean +- SD of three ELISA determinations. * P < 0.01, compared to the two groups. NS, not significant.

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 21 publications 21 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Rat

Host / Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

RPA-T8

Conjugate

eFluor® 450

Excitation/Emission Profile

View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_1272062

Product Specific Information

Description: The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells.

Applications Reported: This RPA-T8 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This RPA-T8 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD8; CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8b; Leu-2; Leu2 T-lymphocyte antigen; MAL; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

Gene Aliases: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

UniProt ID: (Human) P01732, (Human) P10966

Entrez Gene ID: (Human) 925, (Human) 926

Molecular Function: cytokine receptor defense/immunity protein immunoglobulin receptor superfamily receptor