Invitrogen

CD8a Monoclonal Antibody (RPA-T8), eFluor™ 450, eBioscience™

41 Published Figures

29 References

View all (475) CD8 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (1)
Published Figures (41)

FIGURE: 1 / 42

CD8a Antibody (48-0088-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD3 APC (Product # 17-0038-42) and 0.25 µg of Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (left) or Anti-Human CD8a eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

CD8a Antibody in Immunohistochemistry (IHC) — Figure 2 Immunohistochemical assay of immune cells in tumor tissues before and immediately after RFA. Before RFA: (a) CD4+ cells, (d) CD8+ cells, (g) CD3+ cells, and (j) NK cells. Immediately after RFA: (b) CD4+ cells, (e) CD8+ cells, (h) CD3+ cells, and (k) NK cells. Quantitative data: (c) CD4+ cells, (f) CD8+ cells, (i) CD3+ cells, and (l) NK cells. Data are presented as mean +- SEM. The brown cells are positive cells. Bar = 100 mum. RFA: radiofrequency ablation, CD: cluster of differentiation, NK: natural killer, SEM: standard error of the mean.

Product Details

48-0088-42

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 28 publications 28 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Rat

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

RPA-T8

Conjugate

eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_1272062

Product Specific Information

Description: The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells.

Applications Reported: This RPA-T8 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This RPA-T8 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

Panel Builder

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Severe T cell hyporeactivity in ventilated COVID-19 patients correlates with prolonged virus persistence and poor outcomes.

Nature communications

Renner K,Schwittay T,Chaabane S,Gottschling J,Müller C,Tiefenböck C,Salewski JN,Winter F,Buchtler S,Balam S,Malfertheiner MV,Lubnow M,Lunz D,Graf B,Hitzenbichler F,Hanses F,Poeck H,Kreutz M,Orsó E,Burkhardt R,Niedermair T,Brochhausen C,Gessner A,Salzberger B,Mack M

Published figure using CD8a monoclonal antibody (Product # 48-0088-42) in Flow Cytometry

Fri May 21 00:00:00 EDT 2021

Human endoglin-CD3 bispecific T cell engager antibody induces anti-tumor effect in vivo.

Theranostics

Zhong L,Shi W,Gan L,Liu X,Huo Y,Wu P,Zhang Z,Wu T,Peng H,Huang Y,Zhao Y,Yuan Y,Deng Z,Tang H

Published figure using CD8a monoclonal antibody (Product # 48-0088-42) in Flow Cytometry

Wed Jul 28 00:00:00 EDT 2021

Changes of immune cells in patients with hepatocellular carcinoma treated by radiofrequency ablation and hepatectomy, a pilot study.

Open life sciences

Yu Z,Li G,Yu H,Asakawa T

Published figure using CD8a monoclonal antibody (Product # 48-0088-42) in Immunohistochemistry

Fri Feb 18 00:00:00 EST 2022

Multipeptide stimulated PBMCs generate T_EM/T_CM for adoptive cell therapy in multiple myeloma.

Oncotarget

Vardam-Kaur T,Pathangey LB,McCormick DJ,Bergsagel PL,Cohen PA,Gendler SJ

Published figure using CD8a monoclonal antibody (Product # 48-0088-42) in Flow Cytometry

Tue Sep 28 00:00:00 EDT 2021

Ex vivo expansion of dysfunctional regulatory T lymphocytes restores suppressive function in Parkinson's disease.

NPJ Parkinson's disease

Thome AD,Atassi F,Wang J,Faridar A,Zhao W,Thonhoff JR,Beers DR,Lai EC,Appel SH

48-0088-42 was used in Flow Cytometry to document a shift towards a pro-inflammatory peripheral immune response in patients with Parkinson's disease (PD).

Thu May 13 00:00:00 EDT 2021

A Cross-Reactive Small Protein Binding Domain Provides a Model to Study Off-Tumor CAR-T Cell Toxicity.

Molecular therapy oncolytics

Hammill JA,Kwiecien JM,Dvorkin-Gheva A,Lau VWC,Baker C,Wu Y,Bezverbnaya K,Aarts C,Heslen CW,Denisova GF,Derocher H,Milne K,Nelson BH,Bramson JL

Published figure using CD8a monoclonal antibody (Product # 48-0088-42) in Flow Cytometry

Fri Jun 26 00:00:00 EDT 2020

NKG2C^pos NK Cells Regulate the Expansion of Cytomegalovirus-Specific CD8 T Cells.

Journal of immunology (Baltimore, Md. : 1950)

Grutza R,Moskorz W,Senff T,Bäcker E,Lindemann M,Zimmermann A,Uhrberg M,Lang PA,Timm J,Cosmovici C

48-0088-42 was used in Flow Cytometry to show that NKG2Cpos NK cells interact with HLA-Ehigh CD8 T cells, which may negatively regulate the expansion of CMV-specific CD8 T cells upon activation.

Mon Jun 01 00:00:00 EDT 2020

Logic-Gated ROR1 Chimeric Antigen Receptor Expression Rescues T Cell-Mediated Toxicity to Normal Tissues and Enables Selective Tumor Targeting.

Cancer cell

Srivastava S,Salter AI,Liggitt D,Yechan-Gunja S,Sarvothama M,Cooper K,Smythe KS,Dudakov JA,Pierce RH,Rader C,Riddell SR

48-0088 was used in Flow cytometry/Cell sorting to demonstrate the use of “AND” logic gates as a strategy to increase the portfolio of cell surface molecules on solid tumors that can be targeted safely with CAR-T cells.

Mon Mar 18 00:00:00 EDT 2019

Immunological Properties of Neural Crest Cells Derived from Human Induced Pluripotent Stem Cells.

Stem cells and development

Fujii S,Yoshida S,Inagaki E,Hatou S,Tsubota K,Takahashi M,Shimmura S,Sugita S

Published figure using CD8a monoclonal antibody (Product # 48-0088-42) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2019

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

Published figure using CD8a monoclonal antibody (Product # 48-0088-42) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Detection of Retinal Pigment Epithelium-Specific Antibody in iPSC-Derived Retinal Pigment Epithelium Transplantation Models.

Stem cell reports

Sugita S,Makabe K,Fujii S,Iwasaki Y,Kamao H,Shiina T,Ogasawara K,Takahashi M

Published figure using CD8a monoclonal antibody (Product # 48-0088-42) in Flow Cytometry

Tue Nov 14 00:00:00 EST 2017

Follicular Regulatory CD8 T Cells Impair the Germinal Center Response in SIV and Ex Vivo HIV Infection.

PLoS pathogens

Miles B,Miller SM,Folkvord JM,Levy DN,Rakasz EG,Skinner PJ,Connick E

Published figure using CD8a monoclonal antibody (Product # 48-0088-42) in Flow Cytometry

Sat Oct 01 00:00:00 EDT 2016

Modeling the dynamics of neonatal CD8⁺ T-cell responses.

Immunology and cell biology

Reynaldi A,Smith NL,Schlub TE,Venturi V,Rudd BD,Davenport MP

48008842 was used in flow cytometry to discover cell-intrinsic division and differentiation differences in neonatal CD8+ T cells

Sat Oct 01 00:00:00 EDT 2016

Human Head and Neck Squamous Cell Carcinoma-Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor Cells.

Journal of immunology (Baltimore, Md. : 1950)

Younis RH,Han KL,Webb TJ

Published figure using CD8a monoclonal antibody (Product # 48-0088-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Umbilical Cord-Derived Mesenchymal Stem Cells Suppress Autophagy of T Cells in Patients with Systemic Lupus Erythematosus via Transfer of Mitochondria.

Stem cells international

Chen J,Wang Q,Feng X,Zhang Z,Geng L,Xu T,Wang D,Sun L

Published figure using CD8a monoclonal antibody (Product # 48-0088-42) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

CD28 family of receptors on T cells in chronic HBV infection: Expression characteristics, clinical significance and correlations with PD-1 blockade.

Molecular medicine reports

Tang ZS,Hao YH,Zhang EJ,Xu CL,Zhou Y,Zheng X,Yang DL

Published figure using CD8a monoclonal antibody (Product # 48-0088-42) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Lack of T Cell Response to iPSC-Derived Retinal Pigment Epithelial Cells from HLA Homozygous Donors.

Stem cell reports

Sugita S,Iwasaki Y,Makabe K,Kimura T,Futagami T,Suegami S,Takahashi M

Published figure using CD8a monoclonal antibody (Product # 48-0088-42) in Flow Cytometry

Tue Oct 11 00:00:00 EDT 2016

Cytomegalovirus-Induced Expression of CD244 after Liver Transplantation Is Associated with CD8+ T Cell Hyporesponsiveness to Alloantigen.

Journal of immunology (Baltimore, Md. : 1950)

de Mare-Bredemeijer EL,Shi XL,Mancham S,van Gent R,van der Heide-Mulder M,de Boer R,Heemskerk MH,de Jonge J,van der Laan LJ,Metselaar HJ,Kwekkeboom J

48-0088 was used in Flow cytometry/Cell sorting to suggest that CMV infection restrains CD8(+) T cell alloresponses after LTx.

Sat Aug 15 00:00:00 EDT 2015

Liposomal Glutathione Supplementation Restores TH1 Cytokine Response to Mycobacterium tuberculosis Infection in HIV-Infected Individuals.

Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research

Ly J,Lagman M,Saing T,Singh MK,Tudela EV,Morris D,Anderson J,Daliva J,Ochoa C,Patel N,Pearce D,Venketaraman V

Published figure using CD8a monoclonal antibody (Product # 48-0088-42) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2015

Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.

Nature communications

Miles B,Miller SM,Folkvord JM,Kimball A,Chamanian M,Meditz AL,Arends T,McCarter MD,Levy DN,Rakasz EG,Skinner PJ,Connick E

Published figure using CD8a monoclonal antibody (Product # 48-0088-42) in Flow Cytometry

Tue Oct 20 00:00:00 EDT 2015

RSV-specific airway resident memory CD8+ T cells and differential disease severity after experimental human infection.

Nature communications

Jozwik A,Habibi MS,Paras A,Zhu J,Guvenel A,Dhariwal J,Almond M,Wong EHC,Sykes A,Maybeno M,Del Rosario J,Trujillo-Torralbo MB,Mallia P,Sidney J,Peters B,Kon OM,Sette A,Johnston SL,Openshaw PJ,Chiu C

Published figure using CD8a monoclonal antibody (Product # 48-0088-42) in Flow Cytometry

Mon Dec 21 00:00:00 EST 2015

Investigating the causes for decreased levels of glutathione in individuals with type II diabetes.

PloS one

Lagman M,Ly J,Saing T,Kaur Singh M,Vera Tudela E,Morris D,Chi PT,Ochoa C,Sathananthan A,Venketaraman V

Published figure using CD8a monoclonal antibody (Product # 48-0088-42) in Flow Cytometry

Wed Dec 30 00:00:00 EST 2015

Immune response to JC virus T antigen in patients with and without colorectal neoplasia.

Gut microbes

Butcher LD,Garcia M,Arnold M,Ueno H,Goel A,Boland CR

Published figure using CD8a monoclonal antibody (Product # 48-0088-42) in Flow Cytometry

Tue Jul 01 00:00:00 EDT 2014

CD4+CD25(high), CD8+CD28- cells and thyroid autoantibodies in breast cancer patients.

Central-European journal of immunology

Biylgi O,Karagöz B,Türken O,Gültepe M,Özgün A,Tunçel T,Emirzeoğlu L,Çelik S,Müftüoğlu T,Gökhan Kandemir E

Published figure using CD8a monoclonal antibody (Product # 48-0088-42) in Flow Cytometry

Thu Jul 09 00:00:00 EDT 2015

Ex vivo analysis of resident hepatic pro-inflammatory CD1d-reactive T cells and hepatocyte surface CD1d expression in hepatitis C.

Journal of viral hepatitis

Yanagisawa K,Yue S,van der Vliet HJ,Wang R,Alatrakchi N,Golden-Mason L,Schuppan D,Koziel MJ,Rosen HR,Exley MA

Published figure using CD8a monoclonal antibody (Product # 48-0088-42) in Flow Cytometry

Thu Aug 01 00:00:00 EDT 2013

Proportions of CD4+ memory T cells are altered in individuals chronically infected with Schistosoma haematobium.

Scientific reports

Nausch N,Bourke CD,Appleby LJ,Rujeni N,Lantz O,Trottein F,Midzi N,Mduluza T,Mutapi F

48-0088 was used in Flow cytometry/Cell sorting to investigate the association between human helminth infection and CD4+ memory T cell proportion in individuals chronically infected with Schistosoma haematobium.

Mon Feb 25 00:00:00 EST 2013

Upregulation of programmed death-1 on T cells and programmed death ligand-1 on monocytes in septic shock patients.

Critical care (London, England)

Zhang Y,Li J,Lou J,Zhou Y,Bo L,Zhu J,Zhu K,Wan X,Cai Z,Deng X

Published figure using CD8a monoclonal antibody (Product # 48-0088-42) in Flow Cytometry

Wed Dec 07 00:00:00 EST 2011

Sepsis-induced human lymphocyte apoptosis and cytokine production in "humanized" mice.

Journal of leukocyte biology

Unsinger J,McDonough JS,Shultz LD,Ferguson TA,Hotchkiss RS

Published figure using CD8a monoclonal antibody (Product # 48-0088-42) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2009

B cells and monocytes from patients with active multiple sclerosis exhibit increased surface expression of both HERV-H Env and HERV-W Env, accompanied by increased seroreactivity.

Retrovirology

Brudek T,Christensen T,Aagaard L,Petersen T,Hansen HJ,Møller-Larsen A

Published figure using CD8a monoclonal antibody (Product # 48-0088-42) in Flow Cytometry

Mon Nov 16 00:00:00 EST 2009

Bioinformatics

Protein Aliases: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

Gene Aliases: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

UniProt ID: (Human) P01732, (Human) P10966

Entrez Gene ID: (Human) 925, (Human) 926

Molecular Function: defense/immunity protein immunoglobulin receptor superfamily