Western blot of CD97 in mouse colon (wild-type, left lane and knock-out, right lane) lysate was performed using 35ug protein in RIPA buffer. Lysates were probed with a CD97 polyclonal antibody (Product# PA5-37938) at a dilution of 1ug/ml and incubated for 1 hour, followed by detection using infrared fluorescence (Odyssey).
|Tested species reactivity||Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence C-TSQTRALRSSES, from the C-terminus of mouse Cd97|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.3-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD97 is a 75-85kDa monomeric glycoprotein that is present on the surface of most activated leucocytes. It is a receptor involved in both cell adhesion and signaling. CD97 is a member of the G protein coupled receptor family. It is expressed abundantly in cells of hematopoietic origin and is markedly upregulated on activated T and B cells. ESTs have been isolated from a wide variety of tissue libraries.
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