|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 14 and 270 of Cdc20 (Uniprot ID#Q12834)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-34775 targets CDC20 in WB application and shows reactivity with Human samples.
The PA5-34775 immunogen is recombinant fragment corresponding to a region within amino acids 14 and 270 of Cdc20 (Uniprot ID#Q12834).
Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: bA276H19.3; CDC20 cell division cycle 20 homolog; CDC20A; cell division cycle 20 homolog; Cell division cycle protein 20 homolog; p55CDC
Gene Aliases: bA276H19.3; CDC20; CDC20A; p55CDC
UniProt ID: (Human) Q12834
Entrez Gene ID: (Human) 991
Molecular Function: enzyme modulator
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.