Proteintech

CDC37 Polyclonal Antibody

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CDC37 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of MCF-7 cells, using CDC37 antibody 10218-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

CDC37 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 10218-1-AP ( CDC37 Antibody) at dilution of 1:50 (under 40x lens).

CDC37 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 10218-1-AP (CDC37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CDC37 Antibody in Flow Cytometry (Flow) — 1X10^6 MCF-7 cells were stained with 0.2ug CDC37 antibody (10218-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

CDC37 Antibody in Immunoprecipitation (IP) — IP result of anti-CDC37 (IP:10218-1-AP, 4ug; Detection:10218-1-AP 1:1000) with HEK-293 cells lysate 3200ug.

Product Details

10218-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:20-1:200	-
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Non-human primate
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CDC37 Fusion Protein Ag0267 (1-209 aa encoded by BC000083) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MVDYSVWDH IEVSDDEDET HPNIDTASLF RWRHQARVER MEQFQKEKEE LDRGCRECKR KVAECQRKLK ELEVAEGGKA ELERLQAEAQ QLRKEERSWE QKLEEMRKKE KSMPWNVDTL SKDGFSKSMV NTKPEKTEED SEEVREQKHK TFVEKYEKQI KHFGMLRRWD DSQKYLSDNV HLVCEETANY LVIWCIDLEV EEKCALMEQV (1-209 aa encoded by BC000083)

Target Information

In S. cerevisiae, Cdc37 has been shown to be an important regulator of cell division cycle through its action on cyclin-dependent kinases (CDK's). Cdc37 has also been associated with signaling pathways and kinases unrelated to cell cycle control, including v-Src, casein kinase II, MPS1 kinase and the sevenless receptor tyrosine kinase. Interestingly, HSP90 has also been shown to be associated with many of the same cellular targets as Cdc37. It has now been shown that Cdc37 is the same protein previously described as p50 which has been found to be associated with many of the same proteins as HSP90. Among others, Cdc37/p50 has been found complexed with Src-family kinases (Fyn, Yes, Fes, Lck & Fgr), Raf and pp60v-src. More recently, Cdc37 has been shown to be a chaperone independently and in concert with HSP90. In fact, Cdc37 is necessary for the binding of HSP90 to Cdk4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Non-canonical roles of PFKFB3 in regulation of cell cycle through binding to CDK4.

Oncogene

Jia W,Zhao X,Zhao L,Yan H,Li J,Yang H,Huang G,Liu J

Thu Mar 01 00:00:00 EST 2018

Bioinformatics

Protein Aliases: CDC 37; CDC37 (cell division cycle 37, S. cerevisiae, homolog); CDC37 cell division cycle 37 homolog; Cdc37 homolog; CDC37 protein; cell division cycle 37 homolog; Hsp90 chaperone protein kinase-targeting subunit; Hsp90 co-chaperone Cdc37; Hsp90 co-chaperone Cdc37, N-terminally processed; p50Cdc37

Gene Aliases: CDC37; CDC37A; p50; P50CDC37

UniProt ID: (Human) Q16543, (Mouse) Q61081

Entrez Gene ID: (Human) 11140, (Mouse) 12539

Molecular Function: chaperone