Proteintech

CDC45L Polyclonal Antibody

CDC45L Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 16

CDC45L Antibody (15678-1-AP) in ICC/IF

Immunofluorescent analysis of Hela cells, using CDC45L antibody 15678-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

CDC45L Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CDC45L Antibody in Flow Cytometry (Flow)

CDC45L Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

15678-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CDC45L Fusion Protein Ag8150 (217-566 aa encoded by BC010022)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.31 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This is a rabbit polyclonal antibody raised against part of C-terminus of human CDC45L.

Immunogen sequence: LWWAIVGLT DQWVQDKITQ MKYVTDVGVL QRHVSRHNHR NEDEENTLSV DCTRISFEYD LRLVLYQHWS LHDSLCNTSY TAARFKLWSV HGQKRLQEFL ADMGLPLKQV KQKFQAMDIS LKENLREMIE ESANKFGMKD MRVQTFSIHF GFKHKFLASD VVFATMSLME SPEKDGSGTD HFIQALDSLS RSNLDKLYHG LELAKKQLRA TQQTIASCLC TNLVISQGPF LYCSLMEGTP DVMLFSRPAS LSLLSKHLLK SFVCSTKNRR CKLLPLVMAA PLSMEHGTVT VVGIPPETDS SDRKNFFGRA FEKAAESTSS RMLHNHFDLS VIELKAEDRS KFLDALISLL S (217-566 aa encoded by BC010022)

Target Information

The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple polyadenylation sites of this gene are reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

LSD1 is required for euchromatic origin firing and replication timing.

Signal transduction and targeted therapy

Wang Y,Huang Y,Cheng E,Liu X,Zhang Y,Yang J,Young JTF,Brown GW,Yang X,Shang Y

Wed Apr 13 00:00:00 EDT 2022

The CRL4DTL E3 ligase induces degradation of the DNA replication initiation factor TICRR/TRESLIN specifically during S phase.

Nucleic acids research

Wittig KA,Sansam CG,Noble TD,Goins D,Sansam CL

Mon Oct 11 00:00:00 EDT 2021

Nine hub genes related to the prognosis of HBV-positive hepatocellular carcinoma identified by protein interaction analysis.

Annals of translational medicine

Xie W,Wang B,Wang X,Hou D,Su H,Huang H

Wed Apr 01 00:00:00 EDT 2020

The synthetic antihyperlipidemic drug potassium piperate selectively kills breast cancer cells through inhibiting G1-S-phase transition and inducing apoptosis.

Oncotarget

Fan L,Cao X,Yan H,Wang Q,Tian X,Zhang L,He X,Borjihan G

Tue Jul 18 00:00:00 EDT 2017

The synthetic antihyperlipidemic drug potassium piperate selectively kills breast cancer cells through inhibiting G1-S-phase transition and inducing apoptosis.

Oncotarget

Fan L,Cao X,Yan H,Wang Q,Tian X,Zhang L,He X,Borjihan G

Tue Jul 18 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: CDC 45; CDC 45L; CDC45 cell division cycle 45 homolog; CDC45 like; CDC45-related protein; Cell division control protein 45 homolog; cell division cycle 45 homolog; cell division cycle 45-like 2; human CDC45; PORC PI 1; PORC-PI-1

View more View less

Gene Aliases: CDC45; CDC45L; CDC45L2; PORC-PI-1; UNQ374/PRO710

View more View less

UniProt ID: (Human) O75419, (Mouse) Q9Z1X9

View more View less

Entrez Gene ID: (Human) 8318, (Mouse) 12544

View more View less

Function(s)

chromatin binding DNA replication origin binding single-stranded DNA binding protein binding 3'-5' DNA helicase activity replication origin binding protein

Process(es)

DNA replication checkpoint G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle double-strand break repair via break-induced replication DNA replication pre-replicative complex assembly involved in nuclear cell cycle DNA replication DNA replication initiation regulation of chromatin silencing at telomere DNA duplex unwinding positive regulation of G1/S transition of mitotic cell cycle mitotic DNA replication preinitiation complex assembly cell cycle

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CDC45L Polyclonal Antibody

CDC45L Antibody (15678-1-AP) in ICC/IF

Product Details

15678-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!