Invitrogen

CDCA8 Polyclonal Antibody

Product Image Gallery

CDCA8 Antibody in Western Blot (WB) — Western blot analysis of CDCA8 in VERO cell lysate (left lane: treated with blocking peptide). Samples were incubated with CDCA8 polyclonal antibody (Product # PA5-102318).

CDCA8 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded CDCA8 in rat heart tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with CDCA8 polyclonal antibody (Product # PA5-102318) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

CDCA8 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of CDCA8 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and CDCA8 polyclonal antibody (Product # PA5-102318) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC)

1:100-1:500

Immunofluorescence (IF)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human CDCA8, corresponding to a region within N-terminal amino acids.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol, 150mM NaCl

Contains

0.02% sodium azide

Storage conditions

-20°C

RRID

AB_2851727

Target Information

CDCA8 is a component of a chromosomal passenger complex (CPC) required for stability of the bipolar mitotic spindle. The chromosomal passenger complex, which includes Survivin, CDCA8, INCENP and Aurora-B, is known to play crucial roles during mitosis and cell division. It was found that CDCA8 interacting with Aurora-B, INCENP and Survivin, increases during G2/M phase and then reduces after exit from M phase. CDCA8 is cell cycle regulated, down-regulated in response to p53/Rb-signaling, and up-regulated in many types of cancerous tissues. In Drosophila cells, inactivation of CDCA8 results in polyploidy, delayed mitosis and abnormal tissue development, indicating its critical role for cell proliferation. Recent studies show that CDCA8 is essential for cell proliferation during early embryonic development, and its early embryonic lethality cannot be rescued by the loss of p53. Its aberrant expression is linked to a poor prognosis for gastric cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Borealin; Cell division cycle-associated protein 8; Dasra B; Dasra-B; embryonic stem cell-related; hDasra-B; MESrg; Pluripotent embryonic stem cell-related gene 3 protein

Gene Aliases: 4831429J16Rik; AU044747; BOR; BOREALIN; CDCA8; D4Ertd421e; DasraB; MESRGP; PESCRG3

UniProt ID: (Human) Q53HL2, (Rat) Q6AXW0, (Mouse) Q8BHX3

Entrez Gene ID: (Human) 55143, (Rat) 500545, (Mouse) 52276

Function(s)

sister chromatid cohesion mitotic metaphase plate congression protein sumoylation chromosome organization cell division mitotic nuclear division cell cycle

Process(es)

protein binding