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Invitrogen
CDCA8 Polyclonal Antibody
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FIGURE: 1 / 1
CDCA8 Antibody (PA5-90486) in WB
Western blot analysis of CDCA8 in extracts of various cell lines. Samples were incubated with CDCA8 Polyclonal antibody (Product # PA5-90486) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-90486
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-124 of human CDCA8 (NP_0605711)
Conjugate
Form
Liquid
Concentration
1.88 mg/mL
Amount
188 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MAPRKGSSRV AKTNSLRRRK LASFLKDFDR EVEIRIKQIE SDRQNLLKEV DNLYNIEILR LPKALREMNW LDYFALGGNK QALEEAATAD LDITEINKLT AEAIQTPLKS AKTRKVIQVD EMIV; Positive Samples: A-549, 293T, Mouse testis, Rat brain; Cellular Location: Chromosome, Cytoplasm, Nucleus, centromere, cytoskeleton, nucleolus, spindle
Target Information
CDCA8 is a component of a chromosomal passenger complex (CPC) required for stability of the bipolar mitotic spindle. The chromosomal passenger complex, which includes Survivin, CDCA8, INCENP and Aurora-B, is known to play crucial roles during mitosis and cell division. It was found that CDCA8 interacting with Aurora-B, INCENP and Survivin, increases during G2/M phase and then reduces after exit from M phase. CDCA8 is cell cycle regulated, down-regulated in response to p53/Rb-signaling, and up-regulated in many types of cancerous tissues. In Drosophila cells, inactivation of CDCA8 results in polyploidy, delayed mitosis and abnormal tissue development, indicating its critical role for cell proliferation. Recent studies show that CDCA8 is essential for cell proliferation during early embryonic development, and its early embryonic lethality cannot be rescued by the loss of p53. Its aberrant expression is linked to a poor prognosis for gastric cancer.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Borealin; Cell division cycle-associated protein 8; Dasra B; Dasra-B; embryonic stem cell-related; hDasra-B; HPESCRG3; MESrg; Pluripotent embryonic stem cell-related gene 3 protein; unnamed protein product
Gene Aliases: 4831429J16Rik; AU044747; BOR; BOREALIN; CDCA8; D4Ertd421e; DasraB; MESRGP; PESCRG3
UniProt ID: (Human) Q53HL2, (Rat) Q6AXW0, (Mouse) Q8BHX3
Entrez Gene ID: (Human) 55143, (Rat) 500545, (Mouse) 52276
mitotic nuclear division
mitotic metaphase plate congression
chromosome organization
cell division
mitotic sister chromatid segregation
mitotic cell cycle
mitotic cytokinesis
mitotic spindle midzone assembly
positive regulation of mitotic cell cycle spindle assembly checkpoint
mitotic spindle assembly
positive regulation of mitotic sister chromatid separation
positive regulation of attachment of mitotic spindle microtubules to kinetochore
positive regulation of mitotic cytokinesis
cell cycle
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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