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Invitrogen
CDK2 Polyclonal Antibody
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FIGURE: 1 / 4
CDK2 Antibody (PA5-120042) in IHC (P)
Immunohistochemistry analysis of CDK2 in paraffin-embedded human esophageal cancer. Samples were incubated with CDK2 Polyclonal antibody (Product # PA5-120042) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Product Details
PA5-120042
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-298 of human CDK2 (NP_001789.2).
Conjugate
Form
Liquid
Concentration
2.47 mg/mL
Amount
247 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: 293T, HeLa. The target is usually found in the following locations: Cajal body, Cytoplasm, Endosome, Nucleus, centrosome, cytoskeleton, microtubule organizing center.
Immunogen sequence: RALFPGDSEI DQLFRIFRTL GTPDEVVWPG VTSMPDYKPS FPKWARQDFS KVVPPLDEDG RSLLSQMLHY DPNKRISAKA ALAHPFFQDV TKPVPHLRL
Target Information
CDK2/cyclinO is a member of the cyclin dependent protein kinase family complexed to CyclinO. CyclinO was used as a specific protein isoform name for the UNG gene which is localized exclusively in the nucleus. In addition, CyclinO was reported to shows significant uracil-DNA glycosylase activity. CDK2/cyclinO protein levels increase during G1 phase and the protein is turned over during the course of cell cycle. The levels of cyclin A-cdk2 is maximal at the G1/S transition and both cdk2 and cyclin A are found associated with DNA in the initiation complex during replication. Rb protein acts as substrate for cdk2-cyclin E and/or cdk2-cyclin A in vivo. Cdk2 is activated and specifically localized to the nucleus during late G1-, S-, and G2-phase.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cdc2-related protein kinase; CDC28; CDC2A; CDK1; CDK2; Cell division protein kinase 2; Cyclin-dependent kinase 2; EC 2.7.11.22; EC 2.7.11.23; kinase Cdc2; MPF; p33 protein kinase; p33CDK2; p34 protein kinase; unnamed protein product
Gene Aliases: CDK2; CDKN2; p33(CDK2)
UniProt ID: (Human) P24941
Entrez Gene ID: (Human) 1017
nucleotide binding
magnesium ion binding
protein kinase activity
protein serine/threonine kinase activity
cyclin-dependent protein serine/threonine kinase activity
protein binding
ATP binding
kinase activity
transferase activity
protein domain specific binding
cyclin binding
histone kinase activity
metal ion binding
cyclin-dependent protein kinase activity
protein serine kinase activity
G1/S transition of mitotic cell cycle
G2/M transition of mitotic cell cycle
negative regulation of transcription from RNA polymerase II promoter
telomere maintenance
DNA replication
DNA repair
chromatin remodeling
transcription, DNA-templated
protein phosphorylation
potassium ion transport
cellular response to DNA damage stimulus
centriole replication
signal transduction
Ras protein signal transduction
regulation of mitotic cell cycle
positive regulation of cell proliferation
regulation of G2/M transition of mitotic cell cycle
regulation of gene expression
peptidyl-serine phosphorylation
positive regulation of heterochromatin assembly
mitotic G1 DNA damage checkpoint
protection from non-homologous end joining at telomere
positive regulation of DNA-dependent DNA replication initiation
telomere maintenance in response to DNA damage
post-translational protein modification
positive regulation of DNA replication
positive regulation of transcription, DNA-templated
centrosome duplication
cell division
meiotic cell cycle
cellular response to nitric oxide
cellular senescence
regulation of anaphase-promoting complex-dependent catabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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