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Invitrogen
CDK2 Polyclonal Antibody
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FIGURE: 1 / 3
CDK2 Antibody (PA5-77908) in WB
Western blot was performed using Anti-CDK2 Polyclonal Antibody (Product # PA5-77908) and 33 kDa band corresponding to CDK2 was observed across the cell lines tested and increased upon Hydroxyurea treatment in HCT 116 cells. Whole cell extracts (30 µg lysate) of HCT 116 (Lane 1), HCT 116 treated with Hydroxyurea (10mM for 18 hours) (Lane 2), HEK-293 (Lane 3), K-562 (Lane 4), Jurkat (Lane 5) and A-431 (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a n... View More
Product Details
PA5-77908
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human CDK2. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.3 mg/mL
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
Positive Control: 293T, HeLa, HepG2
Predicted Reactivity: Chicken (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
CDK2/cyclinO is a member of the cyclin dependent protein kinase family complexed to CyclinO. CyclinO was used as a specific protein isoform name for the UNG gene which is localized exclusively in the nucleus. In addition, CyclinO was reported to shows significant uracil-DNA glycosylase activity. CDK2/cyclinO protein levels increase during G1 phase and the protein is turned over during the course of cell cycle. The levels of cyclin A-cdk2 is maximal at the G1/S transition and both cdk2 and cyclin A are found associated with DNA in the initiation complex during replication. Rb protein acts as substrate for cdk2-cyclin E and/or cdk2-cyclin A in vivo. Cdk2 is activated and specifically localized to the nucleus during late G1-, S-, and G2-phase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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