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Bethyl Laboratories
CDK4 Polyclonal Antibody
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Product Details
A304-225A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 253 to 303 of human Cyclin-Dependent Kinase 4.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
CDK4/cyclin D3 serine/threonine kinase is dimeric protein complex. CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of Rb protein leading to an active transcriptional repression of E2F complex. CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDK4; Cell division protein kinase 4; Cyclin-dependent kinase 4; MGC14458; orf; PSK-J3; unnamed protein product
Gene Aliases: CDK4; CMM3; PSK-J3
UniProt ID: (Human) P11802
Entrez Gene ID: (Human) 1019
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
cyclin-dependent protein serine/threonine kinase activity
protein binding
ATP binding
kinase activity
cyclin-dependent protein serine/threonine kinase regulator activity
transferase activity
cyclin binding
protein serine kinase activity
G1/S transition of mitotic cell cycle
signal transduction
positive regulation of cell proliferation
response to xenobiotic stimulus
regulation of G2/M transition of mitotic cell cycle
regulation of gene expression
positive regulation of G2/M transition of mitotic cell cycle
positive regulation of fibroblast proliferation
cell division
regulation of cell cycle
regulation of transcription initiation from RNA polymerase II promoter
regulation of type B pancreatic cell proliferation
cellular response to lipopolysaccharide
cellular response to interleukin-4
cellular response to phorbol 13-acetate 12-myristate
cellular response to ionomycin
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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