Proteintech

CDK6 Polyclonal Antibody

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CDK6 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 14052-1-AP (CDK6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

CDK6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human endometrial cancer tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

CDK6 Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 14052-1-AP ( CDK6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CDK6 Antibody in Immunoprecipitation (IP) — IP result of anti-CDK6 (IP:14052-1-AP, 4ug; Detection:14052-1-AP 1:1000) with Jurkat cells lysate 2880 ug.

Product Details

14052-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 24 publications 24 publications
Immunohistochemistry (IHC)	-	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:500	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Miscellaneous PubMed (Misc)	-	View 3 publications 3 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Zebrafish
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CDK6 Fusion Protein Ag5275 (1-326 aa encoded by BC027989) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.17 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MEKDGLCRA DQQYECVAEI GEGAYGKVFK ARDLKNGGRF VALKRVRVQT GEEGMPLSTI REVAVLRHLE TFEHPNVVRL FDVCTVSRTD RETKLTLVFE HVDQDLTTYL DKVPEPGVPT ETIKDMMFQL LRGLDFLHSH RVVHRDLKPQ NILVTSSGQI KLADFGLARI YSFQMALTSV VVTLWYRAPE VLLQSSYATP VDLWSVGCIF AEMFRRKPLF RGSSDVDQLG KILDVIGLPG EEDWPRDVAL PRQAFHSKSA QPIEKFVTDI DELGKDLLLK CLTFNPAKRI SAYSALSHPY FQDLERCKEN LDSHLPPSQN TSELNTA (1-326 aa encoded by BC027989)

Target Information

Cyclin-dependent kinase 6 (p40cdk6 also known as PLSTIRE) belongs to a family of proteins designated as cdks. They are critical regulators of cell cycle progression. The prototype member of this family, p34cdc2, and a related protein cdk2, function late in the cycle while cdk4 and cdk6 are critically involved in G1 to S progression. CDK6 is essential for cell proliferation within the dentate gyrus of the hippocampus and the cuventricular zone of the lateral ventricles. Mutations in the gene results in microcephaly 12, primary, autosomal recessive (MCPH12).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cell division protein kinase 6; CR2 protein kinase; CRK2; Cyclin-dependent kinase 6; MGC59692; Serine/threonine-protein kinase PLSTIRE

Gene Aliases: 5830411I20; AI504062; CDK6; CDKN6; Crk2; MCPH12; PLSTIRE

UniProt ID: (Human) Q00534, (Mouse) Q64261

Entrez Gene ID: (Human) 1021, (Rat) 114483, (Mouse) 12571

Molecular Function: non-receptor serine/threonine protein kinase