Bethyl Laboratories

CDK7 Polyclonal Antibody

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CDK7 Antibody in Western Blot (WB) — **Detection of mouse and rat CDK7 by western blot.** *Samples:* Whole cell lysate (50 µg) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. *Antibodies:* Affinity purified rabbit anti-CDK7 antibody A300-405A (lot A300-405A-1) used for WB at 1 µg/ml. *Detection:* Chemiluminescence with an exposure time of 30 seconds.

CDK7 Antibody in Immunohistochemistry (IHC) — **Detection of human CDK7 by immunohistochemistry.** *Sample:* FFPE section of human ovarian carcinoma. *Antibody:* Affinity purified rabbit anti-CDK7 (Cat. No. A300-405A Lot2) used at a dilution of 1:1,000 (1µg/ml). *Detection:* DAB.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

Immunohistochemistry (IHC)

1:500-1:2,000

Immunocytochemistry (ICC)

1:100-1:500

Immunoprecipitation (IP)

2-10 µg/mg lysate

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Between 300 and C-term

Conjugate

Unconjugated

Form

liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

tris citrate/phosphate, pH 7-8

Contains

0.09% sodium azide

Storage conditions

4° C

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Target Information

The protein encoded by this gene is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). This gene may be a target of germline cancer-inducing mutations.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 39 KDa protein kinase; 39 protein kinase; CAK; CDK; CDK-activating kinase; CDK-activating kinase 1; Cell division protein kinase 7; CR4 protein kinase; CRK4; CRK4 PK (CDC2-related-kinase-4 protein kinase); Cyclin-dependent kinase 7; cyclin-dependent kinase 7 (homolog of Xenopus MO15 cdk-activating kinase); cyclin-dependent kinase 7 (MO15 homolog Xenopus laevis cdk-activating kinase); cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase); EC 2.7.11.22; EC 2.7.11.23; homolog of Xenopus MO15 Cdk-activating kinase; kinase subunit of CAK; OTTHUMP00000222032; OTTHUMP00000222033; P39 Mo15; Protein-tyrosine kinase MPK-7; serine/threonine kinase stk1; serine/threonine protein kinase 1; serine/threonine protein kinase MO15; Serine/threonine-protein kinase 1; TFIIH basal transcription factor complex kinase subunit

Gene Aliases: AI323415; AI528512; C230069N13; CAK; CAK1; CDK7; CDKN7; Crk4; HCAK; MO15; Mpk-7; p39MO15; STK1

UniProt ID: (Human) P50613, (Mouse) Q03147

Entrez Gene ID: (Human) 1022, (Rat) 171150, (Mouse) 12572

Molecular Function: kinase non-receptor serine/threonine protein kinase non-receptor tyrosine protein kinase protein kinase transferase