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CDK9 Polyclonal Antibody

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CDK9 Polyclonal Antibody

Product Details

100-401-167

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (IHC)

1:200-1:1,000
-

Immunocytochemistry (ICC/IF)

1:500
-

ELISA (ELISA)

1:10,000-1:50,000
-

Immunoprecipitation (IP)

1:500-1:3,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Multiple synthetic peptides corresponding to C-terminal and N-terminal domains of the protein coded by the human gene cdk9 (PITALRE).
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

75 mg/mL

Amount

7.5 mg

Storage buffer

0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Antiserum will specifically react with a 43 kDa cdk9 (PITALRE) protein from human, rat and mouse tissue. No reaction was observed against other related cyclin dependent kinases. Cross reactivity with cdk9 (PITALRE) from other species may also occur. The murine cDNA is shown to be 98% identical with human. For immunohistochemistry use paraffin embedded tissue.

Target Information

CDK9 is a serine/threonine kinase which when complexed with CyclinT1 forms an active holoenzyme complex. This complex plays a key role in regulating the eukaryotic cell cycle. CDK9 is coexpressed and copurified with CyclinT1. CDK9 regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors. CDK9 promotes RNA synthesis in genetic programs for cell growth, differentiation, and viral pathogenesis. The CDK9/cyclin-K complex also has kinase activity towards CTD of RNAPII and can substitute for CDK9/cyclin-T P-TEFb in vitro. Chronic activation of CDK9 causes cardiac myocyte enlargement leading to cardiac hypertrophy and confers a predisposition to heart failure.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: C-2K; CDC2-related kinase; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; Cyclin-dependent kinase 9; cyclin-dependent kinase 9 (CDC2-related kinase); RP11-228B15.5; serine/threonine protein kinase PITALRE; Serine/threonine-protein kinase PITALRE; Tat-associated kinase complex catalytic subunit; unnamed protein product

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Gene Aliases: C-2k; CDC2L4; CDK9; CTK1; PITALRE; TAK

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UniProt ID: (Human) P50750, (Mouse) Q99J95, (Rat) Q641Z4

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Entrez Gene ID: (Human) 1025, (Mouse) 107951, (Rat) 362110

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Function(s)
nucleotide binding transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription coactivator binding DNA binding chromatin binding obsolete transcription elongation regulator activity protein kinase activity protein serine/threonine kinase activity cyclin-dependent protein serine/threonine kinase activity protein binding ATP binding RNA polymerase II carboxy-terminal domain kinase activity kinase activity transferase activity snRNA binding protein kinase binding 7SK snRNA binding protein serine kinase activity transcription factor binding transferase activity, transferring phosphorus-containing groups transcription regulatory region DNA binding non-receptor serine/threonine protein kinase
Process(es)
DNA repair regulation of DNA repair transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter transcription elongation from RNA polymerase II promoter protein phosphorylation cellular response to DNA damage stimulus regulation of mitotic cell cycle cell proliferation replication fork processing regulation of mRNA 3'-end processing positive regulation of transcription elongation from RNA polymerase II promoter negative regulation of transcription elongation from RNA polymerase II promoter positive regulation by host of viral transcription positive regulation of transcription from RNA polymerase II promoter regulation of muscle cell differentiation nucleus localization regulation of cell cycle cellular response to cytokine stimulus negative regulation of protein localization to chromatin positive regulation of protein localization to chromatin transcription elongation-coupled chromatin remodeling transcription pausing by RNA polymerase II transcription, DNA-templated regulation of transcription, DNA-templated positive regulation of cardiac muscle hypertrophy phosphorylation regulation of histone modification positive regulation of histone phosphorylation replication fork arrest negative regulation of cell cycle arrest negative regulation of mRNA polyadenylation positive regulation of mRNA 3'-UTR binding positive regulation of histone H2B ubiquitination response to drug
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