Invitrogen

CDK9 Polyclonal Antibody

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CDK9 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of CDK9 in 4% paraformaldehyde-fixed HeLa cells using CDK9 polyclonal antibody (Product # PA5-85238) at a dilution of 1:500. Sample was then incubated with phalloidin secondary antibody at a dilution of 1:500.

CDK9 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of CDK9 in paraffin-embedded rat adrenal gland using CDK9 polyclonal antibody (Product # PA5-85238) at a dilution of 1:500.

CDK9 Antibody in Western Blot (WB) — Western blot analysis of CDK9 in A) NIH-3T3 cells using CDK9 polyclonal antibody (Product # PA5-85238) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 10% SDS PAGE.

Product Details

PA5-85238

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein encompassing a sequence within the center region of human CDK9.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol, 1% BSA
Contains	0.01% thimerosal
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2792381

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Mouse (98%), Rat (98%), Zebrafish (86%), Xenopus laevis (85%), Pig (98%), Chicken (87%), Rhesus Monkey (100%), Bovine (98%).

Positive Control: HL-60, NIH-3T3.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

CDK9 (cyclin-dependent kinase 9) is a member of the cyclin-dependent protein kinase family. It is involved in the regulation of transcription. CDK9 is a member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex. This complex facilitates the transition from abortive to productive elongation by phosphorylating the CTD of the large subunit of RNA polymerase II, POLR2A, SUPT5H, and RDBP. The complex is inactive when in the 7SK snRNP complex form. CDK9 phosphorylates EP300, MYOD1, RPB1/POLR2A, AR, DSIF, and NELF. It regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors. CDK9 promotes RNA synthesis in genetic programs for cell growth, differentiation, and viral pathogenesis. The CDK9/cyclin-K complex also has kinase activity towards CTD of RNAPII and can substitute for CDK9/cyclin-T P-TEFb in vitro. Chronic activation of CDK9 causes cardiac myocyte enlargement leading to cardiac hypertrophy and confers a predisposition to heart failure.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: C-2K; CDC2-related kinase; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; Cyclin-dependent kinase 9; cyclin-dependent kinase 9 (CDC2-related kinase); RP11-228B15.5; serine/threonine protein kinase PITALRE; Serine/threonine-protein kinase PITALRE; Tat-associated kinase complex catalytic subunit

Gene Aliases: C-2k; CDC2L4; CDK9; CTK1; PITALRE; TAK

UniProt ID: (Human) P50750, (Mouse) Q3UQI6, (Rat) Q641Z4

Entrez Gene ID: (Human) 1025, (Mouse) 107951, (Rat) 362110

Molecular Function: non-receptor serine/threonine protein kinase