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Invitrogen
CDKN2AIP Polyclonal Antibody
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FIGURE: 1 / 1
CDKN2AIP Antibody (PA5-109507) in WB
Western blot analysis of CDKN2AIP in extracts of various cell lines. Samples were incubated with CDKN2AIP Polyclonal antibody (Product # PA5-109507) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
PA5-109507
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human CDKN2AIP (NP_060102.1).
Conjugate
Form
Liquid
Concentration
0.58 mg/mL
Amount
58 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MAQEVSEYLS QNPRVAAWVE ALRCDGETDK HWRHRRDFLL RNAGDLAPAG GAASASTDEA ADAESGTRNR QLQQLISFSM AWANHVFLGC RYPQKVMDKI LSMAEGIKVT DAPTYTTRDE LVAKVKKRGI SSSNEGVEEP SKKRVIEGKN SSAVEQDHAK TSAKTERASA QQENSSTCIG SAIKSESGNS ARSSGISSQN SSTSDGDRSV SSQSSSSVSS
Target Information
CARF was first cloned as a novel binding partner of ARF from a yeast-interactive screen. CARF and ARF colocalize in the perinucleolar region and have a collaborative function. In the nucleoplasm, CARF interacts with p53 and enhances its function. The p53 downregulates CARF in a negative feedback regulatory loop and may also involve p53 antagonist HDM2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDKN2A-interacting protein; collaborates/cooperates with ARF (alternate reading frame) protein; Collaborator of ARF; unnamed protein product
Gene Aliases: 4921511I16Rik; AW208986; CARF; CDKN2AIP; RGD1305302
UniProt ID: (Human) Q9NXV6, (Mouse) Q8BI72, (Rat) Q5U2X0
Entrez Gene ID: (Human) 55602, (Mouse) 70925, (Rat) 306455
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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