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Invitrogen
CDKN2B Polyclonal Antibody
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FIGURE: 1 / 4
CDKN2B Antibody (PA5-99491) in ICC/IF
Immunofluorescent analysis of CDKN2B in LOVO cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% saponin, blocked with 10% serum (45 min, 37°C) incubated with CDKN2B polyclonal antibody (Product # PA5-99491) using a dilution of 1:400 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Product Details
PA5-99491
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CDKN2B(Accession P42772), corresponding to amino acid residues L93-D138.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total p15 INK4b.
Target Information
The four known 15-to 19 kDa INK4 proteins (p15INK4b, p16INK4a, p18INK4c andp19INK4d) bind and inhibit CDK4 and CDK6 but not other CDKs, including cyclin E-Cdk2, cyclin A-Cdk2 and cyclin B-Cdk1. Like the three D-type cyclins, the INK4 genes are expressed in distinct tissue-specific patterns, suggesting that they are not strictly redundant. 5 p18INK4c is expressed during G1 to S transition in the eukaryotic cell division cycle. It is maximally induced as cells enter S-phase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDK inhibitory protein; CDK4B inhibitor; CDKN2B; Cyclin dependent kinase inhibitor 2B (p15, inhibits CDK4); Cyclin-dependent kinase 4 inhibitor B; cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4); cyclin-dependent kinase inhibitor p15; cyclin-dependent kinase inhibitor p15INK4b; cyclin-dependent kinase inhibitor protein; cyclin-dependent kinases 4 and 6 binding protein; MTS-2; Multiple tumor suppressor 2; p10; p14-INK4b; p14_CDK inhibitor; p14_INK4B; p15; p15 CDK inhibitor; p15-INK4b; p15_INK4B; p15INK4B
Gene Aliases: AV083695; CDK4I; CDKN2B; Ink4; INK4B; MTS2; P15; p15(INK4b); p15INK4b; TP15
UniProt ID: (Human) P42772, (Mouse) P55271, (Rat) P55272
Entrez Gene ID: (Human) 1030, (Mouse) 12579, (Rat) 25164
transforming growth factor beta receptor signaling pathway
negative regulation of cell proliferation
megakaryocyte differentiation
positive regulation of transforming growth factor beta receptor signaling pathway
cellular response to nutrient
spleen development
negative regulation of epithelial cell proliferation
regulation of G0 to G1 transition
cellular response to cell-matrix adhesion
cellular senescence
regulation of G1/S transition of mitotic cell cycle
negative regulation of G1/S transition of mitotic cell cycle
regulation of cyclin-dependent protein serine/threonine kinase activity
G2/M transition of mitotic cell cycle
cell cycle
cell cycle arrest
mitotic cell cycle checkpoint
cellular response to extracellular stimulus
negative regulation of phosphorylation
negative regulation of protein serine/threonine kinase activity
liver development
aging
response to organic cyclic compound
positive regulation of epithelial cell differentiation
response to cytokine
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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