|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 16 amino acid peptide near the carboxy terminus of human CDX2.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||20 ug/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Immunohistochemistry (IHC)||5 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control is mouse brain tissue lysate.
PA5-20891 can be used with blocking peptide PEP-1005.
CDX2 is a member of the caudal homeobox family of transcription factors that also includes the proteins CDX1 and CDX4. CDX2 is strongly expressed in the trophectoderm in the blastocyst with expression maintained within the proliferating extra-embryonic ectoderm during development. It is required for the repression of the transcription of the POU5F1/Oct4 and NANOG genes in the outer cells of the blastocyst and is thus essential for the segregation of the inner cell mass and trophectoderm at the blastocyst stage. Aberrant expression of CDX2 is associated with numerous types of carcinomas, including intestinal metaplasia, gastric carcinoma, and acute myeloid leukemia due to deregulated HOX gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thyroid and glucocorticoid hormones induce expression of lactase-phlorizin hydrolase gene in CDX-2/HNF-1¿ co-transfected IEC-6 cells.
PA5-20891 was used in western blot to investigate the role of thyroid and glucocorticoid hormones on enzymes needed for lactose digestion
|Suzuki T,Mochizuki K,Goda T||Journal of nutritional science and vitaminology (60:321)||2015|
The myc 3' wnt-responsive element suppresses colonic tumorigenesis.
PA5-20891 was used in ChIP assay to study the suppression of colonic tumorigenesis by the myc 3' Wnt-responsive element
|Konsavage WM,Yochum GS||Molecular and cellular biology (34:1659)||2014|