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Rockland
CEBP delta Polyclonal Antibody
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FIGURE: 1 / 2
CEBP delta Antibody (600-401-A61) in WB
Western Blot of Anti-C/EBPd Antibody. Lane 1: untreated wild-type (WT) cells. Lane 2: WT cells treated 3 h with LPS. Lane 3: C/EBPd-knock-out cells treated 3H with LPS. Load: 35 µg per lane. Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4°C. Blocking: buffer TBST. ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kDa, ~35 kDa.
Product Details
600-401-A61
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the amino terminus of mouse C/EBP.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This antibody recognizes endogenous mouse and recombinant human C/EBP delta protein. A BLAST analysis was used to suggest cross-reactivity with C/EBPd from human sources based on 77% homology with the immunizing sequence. No cross-reactivity is observed with mouse C/EBPa or C/EBPb. Reactivity with C/EBPd from other sources has not been determined.
Target Information
C/EBP delta are CCAAT enhancer binding proteins (CEBPs), a family of transcription factors that all contain a highly conserved, basic-leucine zipper domain at the C-terminus that is involved in dimerization and DNA binding. At least six members of the family have been isolated and characterized to date (CEBP alpha to CEBP zeta). CEBPD is a leucine zipper (LZ) DNA-binding protein that regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. CEBPD is an important transcriptional activator in the regulation of genes involved in immune and inflammatory responses and has been reported to possess many tumor suppressor-like properties.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C EBP; C/EBP delta; C/EBP-related protein 3; CCAAT/enhancer binding protein (C/EBP), delta; CCAAT/enhancer-binding protein delta; NF-IL6-beta; Nuclear factor NF-IL6-beta
Gene Aliases: C/EBP-delta; CEBPD; CELF; CRP3; NF-IL6-beta
UniProt ID: (Human) P49716, (Mouse) Q00322
Entrez Gene ID: (Human) 1052, (Mouse) 12609
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
protein binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein homodimerization activity
sequence-specific DNA binding
transcription regulatory region DNA binding
protein heterodimerization activity
basic leucine zipper transcription factor
transcription from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase II promoter
hematopoietic progenitor cell differentiation
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
fat cell differentiation
positive regulation of osteoblast differentiation
negative regulation of transcription, DNA-templated
inner ear development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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