Proteintech

CENPJ Polyclonal Antibody

Product Image Gallery

CENPJ Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 11517-1-AP (CENPJ antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

CENPJ Antibody in Western Blot (WB) — Mouse testis tissue were subjected to SDS PAGE followed by western blot with 11517-1-AP (CENPJ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Product Details

11517-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 13 publications 13 publications
Immunocytochemistry (ICC/IF)	1:10-1:100	View 24 publications 24 publications
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat, Zebrafish
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CENPJ Fusion Protein Ag2078 (1033-1338 aa encoded by BC024209) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.45 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: RENTDLREE IKVMERFRLD AWKRAEAIES SLEVEKKDKL ANTSVRFQNS QISSGTQVEK YKKNYLPMQG NPPRRSKSAP PRDLGNLDKG QAASPREPLE PLNFPDPEYK EEEEDQDIQG EISHPDGKVE KVYKNGCRVI LFPNGTRKEV SADGKTITVT FFNGDVKQVM PDQRVIYYYA AAQTTHTTYP EGLEVLHFSS GQIEKHYPDG RKEITFPDQT VKNLFPDGQE ESIFPDGTIV RVQRDGNKLI EFNNGQRELH TAQFKRREYP DGTVKTVYAN GHQETKYRSG RIRVKDKEGN VLMDTEL (1033-1338 aa encoded by BC024209)

Target Information

This gene encodes a protein that belongs to the centromere protein family. During cell division, this protein plays a structural role in the maintenance of centrosome integrity and normal spindle morphology, and it is involved in microtubule disassembly at the centrosome. This protein can function as a transcriptional coactivator in the Stat5 signaling pathway, and also as a coactivator of NF-kappaB-mediated transcription, likely via its interaction with the coactivator p300/CREB-binding protein. Mutations in this gene are associated with primary autosomal recessive microcephaly, a disorder characterized by severely reduced brain size and mental retardation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cenpj Regulates Cilia Disassembly and Neurogenesis in the Developing Mouse Cortex.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Ding W,Wu Q,Sun L,Pan NC,Wang X

Wed Mar 13 00:00:00 EDT 2019

Cenpj Regulates Cilia Disassembly and Neurogenesis in the Developing Mouse Cortex.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Ding W,Wu Q,Sun L,Pan NC,Wang X

Wed Mar 13 00:00:00 EDT 2019

Albatross/FBF1 contributes to both centriole duplication and centrosome separation.

Genes to cells : devoted to molecular & cellular mechanisms

Inoko A,Yano T,Miyamoto T,Matsuura S,Kiyono T,Goshima N,Inagaki M,Hayashi Y

Sat Dec 01 00:00:00 EST 2018

PPP1R35 is a novel centrosomal protein that regulates centriole length in concert with the microcephaly protein RTTN.

eLife

Sydor AM,Coyaud E,Rovelli C,Laurent E,Liu H,Raught B,Mennella V

Fri Aug 31 00:00:00 EDT 2018

Centrobin controls primary ciliogenesis in vertebrates.

The Journal of cell biology

Ogungbenro YA,Tena TC,Gaboriau D,Lalor P,Dockery P,Philipp M,Morrison CG

Mon Apr 02 00:00:00 EDT 2018

NEK7 is required for G1 progression and procentriole formation.

Molecular biology of the cell

Gupta A,Tsuchiya Y,Ohta M,Shiratsuchi G,Kitagawa D

Sat Jul 15 00:00:00 EDT 2017

NEK7 is required for G1 progression and procentriole formation.

Molecular biology of the cell

Gupta A,Tsuchiya Y,Ohta M,Shiratsuchi G,Kitagawa D

Sat Jul 15 00:00:00 EDT 2017

Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate.

Neuron

Jayaraman D,Kodani A,Gonzalez DM,Mancias JD,Mochida GH,Vagnoni C,Johnson J,Krogan N,Harper JW,Reiter JF,Yu TW,Bae BI,Walsh CA

Wed Nov 23 00:00:00 EST 2016

Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate.

Neuron

Jayaraman D,Kodani A,Gonzalez DM,Mancias JD,Mochida GH,Vagnoni C,Johnson J,Krogan N,Harper JW,Reiter JF,Yu TW,Bae BI,Walsh CA

Wed Nov 23 00:00:00 EST 2016

Autophagy controls centrosome number by degrading Cep63.

Nature communications

Watanabe Y,Honda S,Konishi A,Arakawa S,Murohashi M,Yamaguchi H,Torii S,Tanabe M,Tanaka S,Warabi E,Shimizu S

Mon Nov 21 00:00:00 EST 2016

Role for the IFT-A Complex in Selective Transport to the Primary Cilium.

Cell reports

Fu W,Wang L,Kim S,Li J,Dynlacht BD

Tue Nov 01 00:00:00 EDT 2016

Cep295 is a conserved scaffold protein required for generation of a bona fide mother centriole.

Nature communications

Tsuchiya Y,Yoshiba S,Gupta A,Watanabe K,Kitagawa D

Fri Aug 26 00:00:00 EDT 2016

Promotion and Suppression of Centriole Duplication Are Catalytically Coupled through PLK4 to Ensure Centriole Homeostasis.

Cell reports

Kim M,O'Rourke BP,Soni RK,Jallepalli PV,Hendrickson RC,Tsou MB

Tue Aug 02 00:00:00 EDT 2016

53BP1 and USP28 mediate p53 activation and G1 arrest after centrosome loss or extended mitotic duration.

The Journal of cell biology

Meitinger F,Anzola JV,Kaulich M,Richardson A,Stender JD,Benner C,Glass CK,Dowdy SF,Desai A,Shiau AK,Oegema K

Mon Jul 18 00:00:00 EDT 2016

Genome-wide screen identifies novel machineries required for both ciliogenesis and cell cycle arrest upon serum starvation.

Biochimica et biophysica acta

Kim JH,Ki SM,Joung JG,Scott E,Heynen-Genel S,Aza-Blanc P,Kwon CH,Kim J,Gleeson JG,Lee JE

Wed Jun 01 00:00:00 EDT 2016

De novo centriole formation in human cells is error-prone and does not require SAS-6 self-assembly.

eLife

Wang WJ,Acehan D,Kao CH,Jane WN,Uryu K,Tsou MF

Thu Nov 26 00:00:00 EST 2015

MDM1 is a microtubule-binding protein that negatively regulates centriole duplication.

Molecular biology of the cell

Van de Mark D,Kong D,Loncarek J,Stearns T

Sun Nov 01 00:00:00 EDT 2015

A Short CEP135 Splice Isoform Controls Centriole Duplication.

Current biology : CB

Dahl KD,Sankaran DG,Bayless BA,Pinter ME,Galati DF,Heasley LR,Giddings TH,Pearson CG

Mon Oct 05 00:00:00 EDT 2015

Plk1 relieves centriole block to reduplication by promoting daughter centriole maturation.

Nature communications

Shukla A,Kong D,Sharma M,Magidson V,Loncarek J

Fri Aug 21 00:00:00 EDT 2015

Plk1 relieves centriole block to reduplication by promoting daughter centriole maturation.

Nature communications

Shukla A,Kong D,Sharma M,Magidson V,Loncarek J

Fri Aug 21 00:00:00 EDT 2015

Cell biology. Reversible centriole depletion with an inhibitor of Polo-like kinase 4.

Science (New York, N.Y.)

Wong YL,Anzola JV,Davis RL,Yoon M,Motamedi A,Kroll A,Seo CP,Hsia JE,Kim SK,Mitchell JW,Mitchell BJ,Desai A,Gahman TC,Shiau AK,Oegema K

Fri Jun 05 00:00:00 EDT 2015

Cenpj/CPAP regulates progenitor divisions and neuronal migration in the cerebral cortex downstream of Ascl1.

Nature communications

Garcez PP,Diaz-Alonso J,Crespo-Enriquez I,Castro D,Bell D,Guillemot F

Tue Mar 10 00:00:00 EDT 2015

Cenpj/CPAP regulates progenitor divisions and neuronal migration in the cerebral cortex downstream of Ascl1.

Nature communications

Garcez PP,Diaz-Alonso J,Crespo-Enriquez I,Castro D,Bell D,Guillemot F

Tue Mar 10 00:00:00 EDT 2015

RBM14 prevents assembly of centriolar protein complexes and maintains mitotic spindle integrity.

The EMBO journal

Shiratsuchi G,Takaoka K,Ashikawa T,Hamada H,Kitagawa D

Fri Jan 02 00:00:00 EST 2015

RBM14 prevents assembly of centriolar protein complexes and maintains mitotic spindle integrity.

The EMBO journal

Shiratsuchi G,Takaoka K,Ashikawa T,Hamada H,Kitagawa D

Fri Jan 02 00:00:00 EST 2015

Stabilization of cartwheel-less centrioles for duplication requires CEP295-mediated centriole-to-centrosome conversion.

Cell reports

Izquierdo D,Wang WJ,Uryu K,Tsou MF

Thu Aug 21 00:00:00 EDT 2014

SAS-6 assembly templated by the lumen of cartwheel-less centrioles precedes centriole duplication.

Developmental cell

Fong CS,Kim M,Yang TT,Liao JC,Tsou MF

Mon Jul 28 00:00:00 EDT 2014

Centrobin-centrosomal protein 4.1-associated protein (CPAP) interaction promotes CPAP localization to the centrioles during centriole duplication.

The Journal of biological chemistry

Gudi R,Zou C,Dhar J,Gao Q,Vasu C

Fri May 30 00:00:00 EDT 2014

Centrobin-centrosomal protein 4.1-associated protein (CPAP) interaction promotes CPAP localization to the centrioles during centriole duplication.

The Journal of biological chemistry

Gudi R,Zou C,Dhar J,Gao Q,Vasu C

Fri May 30 00:00:00 EDT 2014

PIPKIγ targets to the centrosome and restrains centriole duplication.

Journal of cell science

Xu Q,Zhang Y,Xiong X,Huang Y,Salisbury JL,Hu J,Ling K

Sat Mar 15 00:00:00 EDT 2014

PIPKIγ targets to the centrosome and restrains centriole duplication.

Journal of cell science

Xu Q,Zhang Y,Xiong X,Huang Y,Salisbury JL,Hu J,Ling K

Sat Mar 15 00:00:00 EDT 2014

The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis.

Nature cell biology

Zhao H,Zhu L,Zhu Y,Cao J,Li S,Huang Q,Xu T,Huang X,Yan X,Zhu X

Sun Dec 01 00:00:00 EST 2013

The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis.

Nature cell biology

Zhao H,Zhu L,Zhu Y,Cao J,Li S,Huang Q,Xu T,Huang X,Yan X,Zhu X

Sun Dec 01 00:00:00 EST 2013

CEP120 and SPICE1 cooperate with CPAP in centriole elongation.

Current biology : CB

Comartin D,Gupta GD,Fussner E,Coyaud É,Hasegan M,Archinti M,Cheung SW,Pinchev D,Lawo S,Raught B,Bazett-Jones DP,Lüders J,Pelletier L

Mon Jul 22 00:00:00 EDT 2013

CEP120 and SPICE1 cooperate with CPAP in centriole elongation.

Current biology : CB

Comartin D,Gupta GD,Fussner E,Coyaud É,Hasegan M,Archinti M,Cheung SW,Pinchev D,Lawo S,Raught B,Bazett-Jones DP,Lüders J,Pelletier L

Mon Jul 22 00:00:00 EDT 2013

A novel tankyrase small-molecule inhibitor suppresses APC mutation-driven colorectal tumor growth.

Cancer research

Lau T,Chan E,Callow M,Waaler J,Boggs J,Blake RA,Magnuson S,Sambrone A,Schutten M,Firestein R,Machon O,Korinek V,Choo E,Diaz D,Merchant M,Polakis P,Holsworth DD,Krauss S,Costa M

Wed May 15 00:00:00 EDT 2013

Tankyrase 1 regulates centrosome function by controlling CPAP stability.

EMBO reports

Kim MK,Dudognon C,Smith S

Wed Aug 01 00:00:00 EDT 2012

Tankyrase 1 regulates centrosome function by controlling CPAP stability.

EMBO reports

Kim MK,Dudognon C,Smith S

Wed Aug 01 00:00:00 EDT 2012

Interaction proteomics identify NEURL4 and the HECT E3 ligase HERC2 as novel modulators of centrosome architecture.

Molecular & cellular proteomics : MCP

Al-Hakim AK,Bashkurov M,Gingras AC,Durocher D,Pelletier L

Fri Jun 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: CENP-J; Centromere protein J; Centrosomal P4.1-associated protein; LAG-3-associated protein; LYST-interacting protein 1; LYST-interacting protein LIP1; LYST-interacting protein LIP7; microcephaly

Gene Aliases: 4932437H03Rik; BM032; CENP-J; CENPJ; CPAP; Gm81; LAP; LIP1; MCPH6; Sas-4; Sas4; SASS4; SCKL4

UniProt ID: (Human) Q9HC77, (Mouse) Q569L8

Entrez Gene ID: (Human) 55835, (Rat) 305909, (Mouse) 219103

Molecular Function: cytoskeletal protein microtubule or microtubule-binding cytoskeletal protein