Immunofluorescent analysis of CEP152 showing staining in the centrosome of HeLa cells. HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 30 min and stained using a CEP152 polyclonal antibody (Product # PA5-34956) diluted at 1:2000. Red: alpha Tubulin antibody (Product # PA5-29281) diluted at 1:5000. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment contains a sequence corresponding to a region within amino acids 58 and 264 of CEP152|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34956 targets CEP152 in WB applications and shows reactivity with Human samples.
The PA5-34956 immunogen is recombinant fragment contains a sequence corresponding to a region within amino acids 58 and 264 of CEP152.
Regulator of genomic integrity and cellular response to DNA damage acting through ATR-mediated checkpoint signaling. Necessary for centrosome duplication. It functions as a molecular scaffold facilitating the interaction of PLK4 and CENPJ, two molecules involved in centriole formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.