Invitrogen

CERT Polyclonal Antibody

View all (15) CERT (GPBP) antibodies

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Datasheet

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CERT Antibody in Western Blot (WB) — Western Blot analysis of CERT (GPBP) using CERT Polyclonal Antibody (Product # PA5-112151) at a dilution of 1:500. Lane A: Hela whole cell lysate, Lane B: A431 whole cell lysate, Lane C: 293T whole cell lysate, (Lysates/proteins at 30 µg per lane). The secondary antibody used was a Goat Anti-Rabbit IgG H&L (Dylight800) at 1:10,000 dilution. Developed using the Odyssey technique and performed under reducing conditions Predicted band size: 71 kDa. Observed band size: 71 kDa (We are unsure as to the identity of these extra bands).

CERT Antibody in Immunoprecipitation (IP) — Immunoprecipitation of CERT (GPBP) was performed on (Lane A) 0.5 mg A431 whole cell lysate and (Lane B) 0.5 mg Hela whole cell lysate, using 0.5 µL of CERT Polyclonal Antibody (Product # PA5-112151) at a dilution of 1:350 and 15 µL of 50 % Protein G agarose. A Dylight 800-labeled antibody to rabbit IgG (H+L) secondary antibody was used at a 1:5000 dilution Developed using the odssey technique and performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 71 kDa.

Product Details

PA5-112151

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunoprecipitation (IP)	0.2-1 µL/mg lysate	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant Human COL4A3BP protein
Conjugate	Unconjugated
Form	Liquid
Purification	Antigen affinity chromatography, Protein A
Storage buffer	PBS
Contains	no preservative
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2866887

Product Specific Information

This product can be used in direct ELISA.

Target Information

CERT (GPBP) is a GC-rich promotor-specific transactivating transcription factor. This protein is suggested to play a role in the development of atherosclerosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Ceramide transfer protein; ceramide transporter; Collagen type IV alpha-3-binding protein; collagen, type IV, alpha 3 (Goodpasture antigen) binding protein; Goodpasture antigen-binding protein; GPBP; hCERT; lipid-transfer protein CERTL; StAR-related lipid transfer (START) domain containing 11; StAR-related lipid transfer protein 11; StARD11; START domain-containing protein 11

Gene Aliases: CERT; CERT1; CERTL; COL4A3BP; GPBP; MRD34; STARD11

UniProt ID: (Human) Q9Y5P4

Entrez Gene ID: (Human) 10087

Molecular Function: transfer/carrier protein