Invitrogen

CERT Polyclonal Antibody

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CERT Antibody in Western Blot (WB) — Western Blot analysis of CERT (GPBP) using CERT Polyclonal Antibody (Product # PA5-112151) at a dilution of 1:500. Lane A: Hela whole cell lysate, Lane B: A431 whole cell lysate, Lane C: 293T whole cell lysate, (Lysates/proteins at 30 µg per lane). The secondary antibody used was a Goat Anti-Rabbit IgG H&L (Dylight800) at 1:10,000 dilution. Developed using the Odyssey technique and performed under reducing conditions Predicted band size: 71 kDa. Observed band size: 71 kDa (We are unsure as to the identity of these extra bands).

CERT Antibody in Immunoprecipitation (IP) — Immunoprecipitation of CERT (GPBP) was performed on (Lane A) 0.5 mg A431 whole cell lysate and (Lane B) 0.5 mg Hela whole cell lysate, using 0.5 µL of CERT Polyclonal Antibody (Product # PA5-112151) at a dilution of 1:350 and 15 µL of 50 % Protein G agarose. A Dylight 800-labeled antibody to rabbit IgG (H+L) secondary antibody was used at a 1:5000 dilution Developed using the odssey technique and performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 71 kDa.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

Immunoprecipitation (IP)

0.2-1 µL/mg lysate

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Human COL4A3BP protein

Conjugate

Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2866887

Product Specific Information

This product can be used in direct ELISA.

Target Information

CERT (GPBP) is a GC-rich promotor-specific transactivating transcription factor. This protein is suggested to play a role in the development of atherosclerosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Ceramide transfer protein; ceramide transporter; Collagen type IV alpha-3-binding protein; collagen, type IV, alpha 3 (Goodpasture antigen) binding protein; Goodpasture antigen-binding protein; GPBP; hCERT; lipid-transfer protein CERTL; StAR-related lipid transfer (START) domain containing 11; StAR-related lipid transfer protein 11; StARD11; START domain-containing protein 11

Gene Aliases: CERT; CERT1; CERTL; COL4A3BP; GPBP; MRD34; STARD11

UniProt ID: (Human) Q9Y5P4

Entrez Gene ID: (Human) 10087

Molecular Function: transfer/carrier protein