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OriGene
LGR5 Monoclonal Antibody (OTI2A2), TrueMAB™
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FIGURE: 1 / 18
LGR5 Antibody (CF503316) in ICC/IF
Figure from citation: [Nature] cells expressing the stem cell marker Lgr5 was detected by immunostaining of hCECs in 2D culture. View Citation
Pubmed id: 27398792
Memo: Human; IF
Pubmed id: 27398792
Memo: Human; IF
Product Details
CF503316
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI2A2
Immunogen
Human recombinant protein fragment corresponding to amino acids 250-550 of human LGR5 produced in HEK293T Cell.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
G protein-coupled receptor GPR49 has been reported to be expressed in brain, skeletal muscle, placenta, and spinal cord. ESTs have been isolated from normal human brain (amygdala), embryo, and placenta libraries and from human germ cell, uterus, and brain libraries. G-protein Coupled Receptors (GPCRs) comprise one of the largest families of signaling molecules with more than a thousand members currently predicted to exist. All GPCRs share a structural motif consisting of seven membrane-spanning helices, and exist in both active and inactive forms. An array of activating ligands participate in the conformation of GPCRs which leads to signaling via G-proteins and downstream effectors. Ongoing studies have also shown the vast series of reactions which participate in the negative regulation of GPCRs. This "turn-off" activity has tremendous implications for the physiological action of the cell, and continues to drive pharmacological research for new drug candidates. Two blockbuster drugs which have been developed as GPCR-targeted pharmaceuticals are Zyprexa (Eli Lilly) and Claritin (Schering-Plough) which have multi-billion dollar shares of the mental health and allergy markets, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: follicle expressed hormone; G protein-coupled receptor 49; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38; leucine-rich repeat containing G protein-coupled receptor 5; leucine-rich repeat-containing G protein-coupled receptor 5; Leucine-rich repeat-containing G-protein coupled receptor 5; orphan G protein-coupled receptor HG38; Orphan G-protein coupled receptor FEX
Gene Aliases: FEX; GPR49; GPR67; GRP49; HG38; LGR5
UniProt ID: (Human) O75473, (Mouse) Q9Z1P4
Entrez Gene ID: (Human) 8549, (Mouse) 14160
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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