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OriGene
NR5A1 Monoclonal Antibody (OTI1A2), TrueMAB™
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FIGURE: 1 / 1
NR5A1 Antibody (CF806209) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR5A1 (RC207577, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-NR5A1. Positive lysates LY417628 (100 µg) and LC417628 (20 µg) can be purchased separately from OriGene.
Product Details
CF806209
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI1A2
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-262 of human NR5A1 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
Steroidogenic factor-1 (SF-1) is an orphan nuclear receptor involved in organ development and differentiation of the endocrine system. SF-1 has been shown to be essential for fetal development of the hypothalamus, pituitary gland, gonads, and the adrenal gland. SF-1 has been shown to promote transcriptional activity of many steroidogenic enzymes from the cytochrome P450 family. SF-1 contains two activation function domains, designated AF1 and AF2, common to many nuclear steroid receptors which have been shown to be necessary for interaction with other nuclear receptors as well as coactivators and corepressors including steroid receptor coactivator-1 (SRC-1), glucocorticoid receptor interacting protein 1 (GRIP1), cAMP response element binding protein (CREB) binding protein (CBP) and silencing mediator of retinoid acid and thyroid hormone receptor (SMRT).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adrenal 4 binding protein; Adrenal 4 binding protein (AD4BP); Adrenal 4-binding protein; Fushi tarazu factor homolog 1; Fushi tarazu factor homolog 1 (FTZ1); nuclear receptor AdBP4; Nuclear receptor subfamily 5 group A member 1; Nuclear receptor subfamily 5 group A member 1 (NR5A1); putative new variant; SF-1; Steroid hormone receptor Ad4BP; Steroidogenic factor 1; Steroidogenic factor 1 (SF1); steroidogenic factor 1 nuclear receptor; steroidogenic factor-1; STF-1; STF1
Gene Aliases: AD4BP; ELP; FTZ1; FTZF1; hSF-1; NR5A1; POF7; SF-1; SF1; SPGF8; SRXX4; SRXY3
UniProt ID: (Human) Q13285
Entrez Gene ID: (Human) 2516
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription cofactor binding
DNA binding
chromatin binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
phospholipid binding
zinc ion binding
lipid binding
enzyme binding
sequence-specific DNA binding
metal ion binding
sequence-specific double-stranded DNA binding
luteinization
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
sex determination
primary sex determination
male gonad development
female gonad development
hormone-mediated signaling pathway
tissue development
positive regulation of gene expression
male sex determination
adrenal gland development
intracellular receptor signaling pathway
Leydig cell differentiation
hormone metabolic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of steroid biosynthetic process
maintenance of protein location in nucleus
Sertoli cell differentiation
response to gonadotropin-releasing hormone
calcineurin-mediated signaling
positive regulation of male gonad development
negative regulation of female gonad development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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