Proteintech

CFLAR/FLIP Polyclonal Antibody

Product Image Gallery

CFLAR/FLIP Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of HeLa cells using 10394-1-AP (CFLAR/FLIP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

CFLAR/FLIP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human skeletal muscle slide using 10394-1-AP ( CFLAR/FLIP antibody at dilution of 1:50.

CFLAR/FLIP Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 10394-1-AP (CFLAR/FLIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CFLAR/FLIP Antibody in Immunoprecipitation (IP) — IP result of anti-CFLAR/FLIP (IP:10394-1-AP, 4 ug; Detection:10394-1-AP 1:500) with Jurkat cells lysate 4000 ug.

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:10-1:100

Immunofluorescence (IF)

1:10-1:100

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunoprecipitation (IP)

0.5-4.0 µg

Western Blot (WB)

1:500-1:2000

View 2 publications 2 publications

Product Specifications

Species

Human

Published species

Human

Expression System

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CFLAR/FLIP Fusion Protein Ag0593

Conjugate

Unconjugated

Form

Liquid

Concentration

0.72 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Target Information

FLIP (CFLAR) is an apoptosis regulator protein which functions as a crucial link between cell survival and cell death pathways in mammalian cells. It acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: c-FLIP; CASH; CASP8 and FADD-like apoptosis regulator; CASP8 and FADD-like apoptosis regulator subunit p12; CASP8 and FADD-like apoptosis regulator subunit p43; Caspase homolog; Caspase-eight-related protein; Caspase-like apoptosis regulatory protein; caspase-related inducer of apoptosis; Casper; Cellular FLICE-like inhibitory protein; CLARP; FADD-like antiapoptotic molecule 1; FLAME-1; I-FLICE; Inhibitor of FLICE; MACH-related inducer of toxicity; MRIT; testis secretory sperm-binding protein Li 233m; Usurpin; usurpin beta

Gene Aliases: c-FLIP; c-FLIPL; c-FLIPR; c-FLIPS; CASH; CASP8AP1; Casper; CFLAR; CLARP; FLAME; FLAME-1; FLAME1; FLIP; I-FLICE; MRIT

UniProt ID: (Human) O15519

Entrez Gene ID: (Human) 8837

Molecular Function: cysteine protease enzyme modulator hydrolase protease protease inhibitor