|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: G(103) R I I A S Y D P D N K E E R(117)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1.0 µg/ml|
|Immunofluorescence (IF)||1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 3 publications below|
PA1-935 detects cystic fibrosis transmembrane conductance factor (CFTR) from cells overexpressing the human protein.
PA1-935 has been successfully used in immunocytochemistry procedures. Immunocytochemical staining of HEK293 cells overexpressing human CFTR with this antibody results in staining primarily of the plasma membrane.
PA1-935 immunizing peptide corresponds to amino acid residues 103-117 from human CFTR protein. This sequence is completely conserved between human, rabbit, and monkey and there is a one amino acid substitution in rat, bovine, and sheep.
Cystic Fibrosis (CF) is a common lethal genetic disease caused by mutations of the gene coding for the cystic fibrosis transmembrane conductance factor, a cAMP regulated chloride channel. Approximately 70% of all CF cases share the deletion of a phenylalanine at position 508 (delta F508) which results in abnormal chloride transport.
Since the CF mutation is lethal, most often by lung and liver disease, it raises the question of why this genetic disease remains as common as it is. One possible explanation is that Salmonella typhi has been shown to use CFTR to enter intestinal epithelial cells and that delta F508 heterozygote and homozygote mice showed 86% and 100% reductions in S. typhi intestinal submucosal uptake.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Incomplete reprogramming after fusion of human multipotent stromal cells and bronchial epithelial cells.
PA1-935 was used in immunocytochemistry to investigate cell function of hybrid cells from genetically tagged multipotent stromal cells and normal human bronchial epithelial cells
|Curril IM,Koide M,Yang CH,Segal A,Wellman GC,Spees JL||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (24:4856)||2010|
Annexin A5 increases the cell surface expression and the chloride channel function of the DeltaF508-cystic fibrosis transmembrane regulator.
PA1-935 was used in immunocytochemistry to study the effect of annexin A5 on the expression and of the deltaF508-cystic fibrosis transmembrane regulator
|Le Drévo MA,Benz N,Kerbiriou M,Giroux-Metges MA,Pennec JP,Trouvé P,Férec C||Biochimica et biophysica acta (1782:605)||2008|
Disruption of CFTR-dependent lipid rafts reduces bacterial levels and corneal disease in a murine model of Pseudomonas aeruginosa keratitis.
PA1-935 was used in immunocytochemistry to study the mechanisms for the entrance of Pseudomonas aeruginosa into human corneal epithelial cells and the ways to disrupt Pseudomonas aeruginosa infection.
|Zaidi T,Bajmoczi M,Zaidi T,Golan DE,Pier GB||Investigative ophthalmology and visual science (49:1000)||2008|