Invitrogen

CGN Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (10) CGN antibodies

Datasheet

Protocols

Questions & Answers

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Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (6)
Advanced Verification (2)

CGN Antibody in Immunohistochemistry (IHC)

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CGN Antibody (PA5-55673) in IHC

Immunohistochemical staining of CGN in human small intestine and lymph node tissues using CGN Polyclonal Antibody (Product # PA5-55673). Corresponding CGN RNA-seq data are presented for the same tissues.

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CGN Antibody in Western Blot (WB) — Western blot analysis of CGN in human cell lines Caco-2 and HeLa using a CGN Polyclonal Antibody (Product # PA5-55673). Corresponding CGN RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

CGN Antibody — Relative expression in western blot: Antibody specificity was demonstrated by detection of known differential basal expression of the target across tissue/cell models. Expression of CGN was observed specifically in CACO-2 cells and in HeLa cells using anti-CGN Polyclonal Antibody (Product # PA5-55673) in western blot. The relative expression levels of CGN within each cell line is shown using RNA-Seq. {RE}

Product Details

PA5-55673

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

Immunohistochemistry (IHC)

1:50-1:200

Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human CGN

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Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2639735

Product Specific Information

Immunogen sequence: DSKQALQQLQ AQLEDYKEKA RREVADAQRQ AKDWASEAEK TSGGLSRLQD EIQRLRQALQ ASQAERDTAR LDKELLAQRL QGLEQEAENK KRSQDDRARQ LKGLEE

Highest antigen sequence identity to the following orthologs: Mouse - 92%, Rat - 92%.

Target Information

Probably plays a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cingulin

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Gene Aliases: CGN; KIAA1319

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UniProt ID: (Human) Q9P2M7

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Entrez Gene ID: (Human) 57530, (Rat) 310655

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Function(s)

transforming growth factor beta receptor signaling pathway biological_process cell-cell adhesion microtubule cytoskeleton organization epithelial cell morphogenesis metabolic process bicellular tight junction assembly

Process(es)

motor activity actin binding protein binding cadherin binding involved in cell-cell adhesion microtubule binding

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CGN Polyclonal Antibody

CGN Antibody (PA5-55673) in IHC

Product Details

PA5-55673

Western Blot (WB)

Immunohistochemistry (IHC)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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