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Invitrogen
CHD7 Polyclonal Antibody
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FIGURE: 1 / 6
CHD7 Antibody (PA5-72964) in ICC/IF
Immunofluorescence analysis of CHD7 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CHD7 Polyclonal Antibody (Product # PA5-72964) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A270... View More
Product Details
PA5-72964
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A genomic peptide made to an N-terminal region of the human Chd7 protein (within residues 25-200).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen has 85% identity to chicken Chd7.
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Target Information
Chromodomain helicase DNA binding protein 7 (CHD7) is a gene located on chromosome 8q12.2 that encodes a member of the chromodomain helicase DNA-binding (CHD) family, which plays a pivotal role in chromatin remodeling and gene expression regulation. CHD7 contains multiple domains that facilitate its involvement in modifying chromatin structure, thereby influencing the accessibility of DNA for transcription factors and other regulatory proteins. The protein is integral to various developmental processes, including neural crest formation, thus affecting tissues such as the nervous system, skeletal structures, and sensory organs. Mutations in CHD7 are associated with CHARGE syndrome, a multisystem disorder characterized by congenital anomalies including coloboma, heart defects, and ear abnormalities. CHD7's role in chromatin dynamics makes it crucial for normal embryonic development and cellular differentiation. Ongoing research is focused on uncovering CHD7's broader implications in developmental biology and its potential as a therapeutic target in chromatin-related disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ATP-dependent chromatin remodeler CHD7; ATP-dependent helicase CHD7; CHARGE association; CHD-7; Chromo domain-containing protein 7; chromodomain-helicase-DNA-binding protein 7; unnamed protein product
Gene Aliases: A730019I05Rik; CHD7; CRG; Cycn; Cyn; Dz; Edy; Flo; GENA 47; Gena 52; GENA 60; HH5; IS3; KAL5; KIAA1416; Lda; metis; Mt; Obt; Todo; WBE1; Whi
UniProt ID: (Human) Q9P2D1, (Mouse) A2AJK6
Entrez Gene ID: (Human) 55636, (Mouse) 320790
nucleotide binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
DNA binding
chromatin binding
protein binding
ATP binding
hydrolase activity
ATPase activity
histone binding
ATP-dependent chromatin remodeler activity
promoter-specific chromatin binding
helicase activity
hydrolase activity, acting on acid anhydrides
skeletal system development
blood vessel development
in utero embryonic development
heart morphogenesis
transcription, DNA-templated
regulation of transcription, DNA-templated
rRNA processing
central nervous system development
adult heart development
sensory perception of sound
locomotory behavior
adult walking behavior
blood circulation
chromatin modification
cranial nerve development
olfactory nerve development
olfactory bulb development
T cell differentiation
female genitalia development
embryonic hindlimb morphogenesis
positive regulation of multicellular organism growth
olfactory behavior
ear morphogenesis
inner ear morphogenesis
camera-type eye development
nose development
semicircular canal morphogenesis
genitalia development
artery morphogenesis
regulation of neurogenesis
cognition
palate development
retina development in camera-type eye
regulation of growth hormone secretion
limb development
face development
epithelium development
blood vessel remodeling
ventricular trabecula myocardium morphogenesis
right ventricular compact myocardium morphogenesis
chromatin organization
chromatin remodeling
transcription from RNA polymerase II promoter
response to bacterium
regulation of gene expression
regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
aorta development
aorta morphogenesis
atrioventricular canal development
chordate embryonic development
positive regulation of transcription from RNA polymerase II promoter
tissue remodeling
neurological system process
regulation of transport
head development
innervation
cardiac septum morphogenesis
secondary palate development
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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