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Invitrogen
CHD8 Polyclonal Antibody
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FIGURE: 1 / 2
CHD8 Antibody (PA5-103741) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded CHD8 in mouse liver tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with CHD8 polyclonal antibody (Product # PA5-103741) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Product Details
PA5-103741
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CHD8(Accession Q9HCK8), corresponding to amino acid residues R2334-V2384.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total CHD8.
Target Information
DNA helicase that acts as a chromatin remodeling factor and regulates transcription. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1-targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating in efficient U6 RNA polymerase III transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent chromatin remodeler CHD8; ATP-dependent helicase CHD8; Axis duplication inhibitor; beta-catenin binding protein; CHD-8; Chromo domain-containing protein 8; chromodomain-helicase-DNA-binding protein 8; Duplin; helicase with SNF2 domain 1
Gene Aliases: 5830451P18Rik; AU015341; AUTS18; CHD8; Duplin; HELSNF1; IDDAM; KIAA1564; mKIAA1564
UniProt ID: (Human) Q9HCK8, (Rat) Q9JIX5, (Mouse) Q09XV5
Entrez Gene ID: (Human) 57680, (Rat) 65027, (Mouse) 67772
nucleotide binding
p53 binding
DNA binding
DNA helicase activity
chromatin binding
protein binding
ATP binding
beta-catenin binding
DNA-dependent ATPase activity
hydrolase activity
ATPase activity
histone binding
histone H3K4me3 reader activity
ATP-dependent chromatin remodeler activity
methylated histone binding
armadillo repeat domain binding
helicase activity
hydrolase activity, acting on acid anhydrides
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
transcription, DNA-templated
brain development
negative regulation of Wnt signaling pathway
DNA duplex unwinding
ATP-dependent chromatin remodeling
negative regulation of apoptotic process
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase III promoter
digestive tract development
canonical Wnt signaling pathway
negative regulation of canonical Wnt signaling pathway
negative regulation of fibroblast apoptotic process
regulation of transcription, DNA-templated
Wnt signaling pathway
chromatin modification
startle response
chromatin organization
chromatin remodeling
mRNA processing
social behavior
prepulse inhibition
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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