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CHKB Antibody (PA5-112385) in ICC/IF
Immunocytochemichal analysis of CHKB in HepG2 cells using a Polyclonal antibody (Product # PA5-112385) at a dilution of 1:100. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were also counter-stained with DAPI.
Product Details
PA5-112385
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human Choline/ethanolamine kinase protein (2-99AA)
Conjugate
Form
Liquid
Concentration
3.34 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Wet ice
RRID
AB_2867120
Target Information
The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. Choline kinase, the initial enzyme in the sequence, plays a role in cell growth proliferation. A related protein, ChoKB (also, known as choline kinase beta), is a 395 amino acid enzyme that catalyzes the phosphorylation of choline by ATP in the presence of magnesium, thereby yielding phosphocholine and ADP. Like all choline kinases, ChoKB possesses ethanoalamine kinase activity and catalyzes the phosphorylation of ethanolamine. The gene encoding ChoKB is located less than 1 kb upstream of the CPT1B gene, suggesting that the ChoKB gene may regulate transcription CPT1B. In mice, mutations and/or deletions in the gene encoding ChoKB are the cause of hindlimb muscular dystrophy and neonatal bone deformity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Choline kinase beta; Choline kinase-like protein; Choline/ethanolamine kinase; choline/ethanolamine kinase beta; choline/ethanolamine kinase-b; choline/ethanolaminekinase; CK; CKB; CKEKB; EK; EKB; Ethanolamine kinase; Ethanolamine kinase beta
Gene Aliases: CHETK; CHKB; CHKL; CK; Ck/Ek; Ck/Ek-beta; CKB; CKEKB; EK; EKB; MDCMC
UniProt ID: (Human) Q9Y259, (Rat) O54783, (Mouse) O55229
Entrez Gene ID: (Human) 1120, (Rat) 29367, (Mouse) 12651
Performance Guarantee
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