Proteintech

CHMP1B Polyclonal Antibody

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CHMP1B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using (Product # 14639-1-AP) (CHMP1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CHMP1B Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 14639-1-AP (CHMP1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

CHMP1B Antibody in Immunoprecipitation (IP) — IP result of anti-CHMP1B (IP:14639-1-AP, 4ug; Detection:14639-1-AP 1:300) with HeLa cells lysate 880ug.

Product Details

14639-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 5 publications 5 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CHMP1B Fusion Protein Ag6222 (1-199 aa encoded by BC065933)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: MSNMEKHLF NLKFAAKELS RSAKKCDKEE KAEKAKIKKA IQKGNMEVAR IHAENAIRQK NQAVNFLRMS ARVDAVAARV QTAVTMGKVT KSMAGVVKSM DATLKTMNLE KISALMDKFE HQFETLDVQT QQMEDTMSST TTLTTPQNQV DMLLQEMADE AGLDLNMELP QGQTGSVGTS VASAEQDELS QRLARLRDQV (1-199 aa encoded by BC065933)

Target Information

CHMP1B belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A, is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 ).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Spastin is required for human immunodeficiency virus-1 efficient replication through cooperation with the endosomal sorting complex required for transport (ESCRT) protein.

Virologica Sinica

Shen W,Liu C,Hu Y,Ding Q,Feng J,Liu Z,Kong X

Thu Jun 01 00:00:00 EDT 2023

Accessory ESCRT-III proteins are conserved and selective regulators of Rab11a-exosome formation.

Journal of extracellular vesicles

Marie PP,Fan SJ,Mason J,Wells A,Mendes CC,Wainwright SM,Scott S,Fischer R,Harris AL,Wilson C,Goberdhan DCI

Wed Mar 01 00:00:00 EST 2023

Spatially resolved phosphoproteomics reveals fibroblast growth factor receptor recycling-driven regulation of autophagy and survival.

Nature communications

Watson J,Ferguson HR,Brady RM,Ferguson J,Fullwood P,Mo H,Bexley KH,Knight D,Howell G,Schwartz JM,Smith MP,Francavilla C

Thu Nov 03 00:00:00 EDT 2022

Vaccinia virus hijacks ESCRT-mediated multivesicular body formation for virus egress.

Life science alliance

Huttunen M,Samolej J,Evans RJ,Yakimovich A,White IJ,Kriston-Vizi J,Martin-Serrano J,Sundquist WI,Frickel EM,Mercer J

Sun Aug 01 00:00:00 EDT 2021

NPC1-mTORC1 Signaling Couples Cholesterol Sensing to Organelle Homeostasis and Is a Targetable Pathway in Niemann-Pick Type C.

Developmental cell

Davis OB,Shin HR,Lim CY,Wu EY,Kukurugya M,Maher CF,Perera RM,Ordonez MP,Zoncu R

Mon Feb 08 00:00:00 EST 2021

NPC1-mTORC1 Signaling Couples Cholesterol Sensing to Organelle Homeostasis and Is a Targetable Pathway in Niemann-Pick Type C.

Developmental cell

Davis OB,Shin HR,Lim CY,Wu EY,Kukurugya M,Maher CF,Perera RM,Ordonez MP,Zoncu R

Mon Feb 08 00:00:00 EST 2021

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

Nature cell biology

Mercier V,Larios J,Molinard G,Goujon A,Matile S,Gruenberg J,Roux A

Sat Aug 01 00:00:00 EDT 2020

Mycobacterium tuberculosis Type VII Secretion System Effectors Differentially Impact the ESCRT Endomembrane Damage Response.

mBio

Mittal E,Skowyra ML,Uwase G,Tinaztepe E,Mehra A,Köster S,Hanson PI,Philips JA

Tue Nov 27 00:00:00 EST 2018

Mycobacterium tuberculosis Type VII Secretion System Effectors Differentially Impact the ESCRT Endomembrane Damage Response.

mBio

Mittal E,Skowyra ML,Uwase G,Tinaztepe E,Mehra A,Köster S,Hanson PI,Philips JA

Tue Nov 27 00:00:00 EST 2018

Mycobacterium tuberculosis Type VII Secretion System Effectors Differentially Impact the ESCRT Endomembrane Damage Response.

mBio

Mittal E,Skowyra ML,Uwase G,Tinaztepe E,Mehra A,Köster S,Hanson PI,Philips JA

Tue Nov 27 00:00:00 EST 2018

Structure of cellular ESCRT-III spirals and their relationship to HIV budding.

eLife

Cashikar AG,Shim S,Roth R,Maldazys MR,Heuser JE,Hanson PI

Fri May 30 00:00:00 EDT 2014

Structure of cellular ESCRT-III spirals and their relationship to HIV budding.

eLife

Cashikar AG,Shim S,Roth R,Maldazys MR,Heuser JE,Hanson PI

Fri May 30 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: Charged multivesicular body protein 1b; Charged multivesicular body protein 1b-1; CHMP1.5; CHMP1b; CHMP1b-1; chromatin modifying protein 1B; Chromatin-modifying protein 1b; Chromatin-modifying protein 1b-1; vacuolar protein sorting 46-2; Vacuolar protein sorting-associated protein 46-2; Vps46-2

Gene Aliases: 2810405I11Rik; C10orf2; C18-ORF2; C18orf2; CHMP1.5; CHMP1B; Chmp1b1; hVps46-2; Vps46-2; Vps46B

UniProt ID: (Human) Q7LBR1, (Mouse) Q99LU0

Entrez Gene ID: (Human) 57132, (Rat) 689364, (Mouse) 67064

Molecular Function: membrane traffic protein