Invitrogen

CHOP Polyclonal Antibody

Advanced Verification
3 Published Figures
6 References
View all (47) CHOP antibodies

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

Datasheet
Protocols
Questions & Answers
Tech Support
Datasheet
Protocols
Questions & Answers
Tech Support
Datasheet
Protocols
Questions & Answers
Tech Support

Product Details

PA5-88116

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
View 3 publications 3 publications

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of
-

Affinity Purification (AP)

-
View 2 publications 2 publications
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

C. elegans

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.38 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2804663

Product Specific Information

Positive Samples: C2C12, C6; Cellular Location: Cytoplasm, Nucleus

Target Information

GADD153 is a small nuclear protein that is capable of dimerizing with transcription factors C/EBP alpha and beta. Once dimerized, this complex inhibits the normal binding and function of C/EBP to classical binding sites. Inversely, the C/EBP GADD153 dimer gains binding activity to other non classical C/EBP stress related targets. Under normal cellular conditions this protein is not expressed in detectable levels, but is highly unregulated during times of cellular/ER stress. Examples of GADD153 inducing stress include: treatment with tunicamycin, nutrient starvation and reducing agents that interfere with the calcium flux across the ER membrane.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: C/EBP homologous protein; C/EBP homoologous protein 10; C/EBP zeta; C/EBP-homologous protein; C/EBP-homologous protein 10; CCAAT/enhancer-binding protein homologous protein; CHOP; CHOP-10; DDIT-3; DNA damage-inducible transcript 3 protein; DNA-damage-inducible transcript 3; Growth arrest and DNA damage-inducible protein GADD153; Growth arrest and DNA-damage-inducible protein GADD153; MGC4154

View more View less

Gene Aliases: CEBPZ; CHOP; CHOP-10; CHOP10; DDIT3; GADD153

View more View less

UniProt ID: (Human) P35638, (Mouse) P35639

View more View less

Entrez Gene ID: (Human) 1649, (Mouse) 13198, (Rat) 29467

View more View less

Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity protein binding transcription factor binding cAMP response element binding protein binding protein homodimerization activity leucine zipper domain binding transcription regulatory region DNA binding protein heterodimerization activity sequence-specific DNA binding RNA polymerase II transcription factor binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter blood vessel maturation transcription, DNA-templated regulation of transcription, DNA-templated apoptotic process ER overload response response to unfolded protein cell cycle cell cycle arrest Wnt signaling pathway endoplasmic reticulum unfolded protein response positive regulation of interleukin-8 production negative regulation of CREB transcription factor activity response to endoplasmic reticulum stress response to starvation mRNA transcription from RNA polymerase II promoter positive regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of DNA binding negative regulation of sequence-specific DNA binding transcription factor activity positive regulation of neuron apoptotic process regulation of transcription involved in anterior/posterior axis specification cell redox homeostasis negative regulation of fat cell differentiation negative regulation of myoblast differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter release of sequestered calcium ion into cytosol negative regulation of protein kinase B signaling intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress negative regulation of canonical Wnt signaling pathway positive regulation of neuron death negative regulation of RNA polymerase II regulatory region sequence-specific DNA binding positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress negative regulation of determination of dorsal identity response to amphetamine cellular response to DNA damage stimulus aging response to nutrient PERK-mediated unfolded protein response ATF6-mediated unfolded protein response response to drug response to hydrogen peroxide regulation of DNA-templated transcription in response to stress embryonic organ development positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway response to oxidative stress
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support